To combat health challenges associated with mosquito-borne diseases, the larvicidal activity of metallic nanoparticles, food-grade polymeric nano-capsules and insecticides was investigated against larvae of Aedes albopictus as an effective alternate control approach. The Ae. albopictus was identified using sequencing and phylogenetic analyses of COXI, CYTB and ITS2 genes. The characterization of synthesized nanostructures was performed through Zetasizer, UV–VIS spectroscopy, atomic force microscopy, scanning electron microscopy and energy dispersive X-ray spectroscopy. The mosquito larvae were exposed to varying concentration of nanostructures and insecticides, and their percentage mortality was evaluated at different time intervals of 24 h and 48 h exposure. The highest efficacy was observed in zinc oxide nanoparticles (ZnO-NPs) and polymeric nanocapsules FG-Cur E-III (LC50 = 0.24 mg/L, LC90 = 0.6 mg/L) and (LC50 = 3.8 mg/L, LC90 = 9.33 mg/L), respectively, after 24 h; while (LC50 = 0.18 mg/L, LC90 = 0.43 mg/L) and (LC50 = 1.95 mg/L, LC90 = 6.46 mg/L), respectively, after 48 h against fourth instar larvae of Ae. albopictus. Ag, CuO, NiTiO3 and CoTiO3 nanoparticles evaluated in this study also showed promising larvicidal activity. Although ZnO-NPs proved to be effective larvicides, their possible toxicity (producing ROS species) can limit their use. The curcumin nanostructures (FG-Cur E-III) stabilized by food-grade materials are thought to exert their larvicidal activity by binding to sterol carrier protein-2, and depriving the larvae from the essential dietary cholesterol, and bears effective larvicidal potential as safe alternative for chemical larvicides, due to their environment friendly, food-grade and easy biodegradability.

为了应对与蚊媒疾病相关的健康挑战，研究了金属纳米颗粒，食品级聚合物纳米胶囊和杀虫剂对白纹伊蚊幼虫的杀幼虫活性，作为一种有效的替代控制方法。该阂。白化病通过对COXI，CYTB和ITS2基因的测序和系统发育分析来鉴定。合成的纳米结构的表征通过Zetasizer，UV-VIS光谱，原子力显微镜，扫描电子显微镜和能量色散X射线光谱进行。将蚊虫幼虫暴露于不同浓度的纳米结构和杀虫剂，并在暴露24小时和48小时的不同时间间隔评估其死亡率。在氧化锌纳米颗粒（ZnO-NPs）和聚合物纳米胶囊FG-Cur E-III中观察到最高功效（LC50 = 0.24 mg / L，LC90 = 0.6 mg / L）和（LC50 = 3.8 mg / L，LC90 = 9.33 24小时后；分别对48龄幼龄幼虫48小时后（LC50 = 0.18 mg / L，LC90 = 0.43 mg / L）和（LC50 = 1.95 mg / L，LC90 = 6.46 mg / L）e 白化病。在这项研究中评估的Ag，CuO，NiTiO3和CoTiO3纳米颗粒还显示出有希望的杀幼虫活性。尽管事实证明ZnO-NP是有效的杀幼虫剂，但其可能的毒性（产生ROS物质）会限制其使用。食品级材料稳定的姜黄素纳米结构（FG-Cur E-III）被认为通过结合固醇载体蛋白2发挥幼虫活性，并从必需的饮食胆固醇中剥夺幼虫，并具有安全有效的幼虫潜能化学杀幼虫剂的替代品，因为它们对环境友好，食品级且易于生物降解。