Researchers have shown that knowledge acquisition and sharing have considerably influenced the acceptance of various technologies. However, there is a scarce of knowledge on how these two factors affect the acceptance of Mobile learning (M-learning). Thus, this research is believed to be one of the few attempts that aims to understand the impact of knowledge acquisition and knowledge sharing on M-learning acceptance through the extension of technology acceptance model (TAM) by these factors. The data were collected from 735 IT undergraduate students enrolled in two different academic institutions in two different developing countries, namely Malaysia and Oman, using questionnaire surveys. The partial least squares-structural equation modeling (PLS-SEM) is used to validate the extended theoretical model. The findings indicated that knowledge acquisition has a significant positive influence on perceived ease of use and perceived usefulness of M-learning in both samples. Moreover, the findings revealed that knowledge sharing has a significant positive impact on perceived usefulness with respect to the Omani sample, whereas this relation was not supported in terms of the Malaysian sample. Theoretical and practical implications, limitations, and future research directions are also discussed.