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How Wheat Pericarp Alter Fungal Growth and Toxigenicity Profiles
Arabian Journal for Science and Engineering ( IF 2.9 ) Pub Date : 2020-11-11 , DOI: 10.1007/s13369-020-05078-1
Mohamed Nasr Gomaa , Omar Abdalhakim Almaghrabi , Awad Abdallah Elshoura , Kawther Mohamed Soliman

Reducing economic losses and health hazards due to mycotoxins without suffering the side effects of chemical fungicides is a goal of many researchers. The hypothesis of the current study is that naturally occurring phytohormones in the pericarp may be responsible for the protective effect of the pericarp against fungal invasion and toxin production. A spore suspension of Aspergillus parasiticus was used to inoculate yeast extract sucrose (YES) medium as a control; an aqueous extract of the pericarp with or without YES medium (PYES or PS) and YES media containing abscisic, jasmonic and gibberellic acids were used to detect the effect of these phytohormones on fungal growth and toxin production. The growth curve was generated on the basis of dry weights, and the aflatoxin concentration was determined by HPLC at each time interval of the study. The aqueous extract of the pericarp (PS) did not support fungal growth or aflatoxin production. However, PYES showed the highest percentages of inhibition of fungal growth (51%) and aflatoxin production (45%) on days 3 and 9, respectively. The reduction in fungal growth due to the presence of phytohormones was over 70% after 3 days and over 14% on day 12; however, no significant difference was observed between the treatments and the control on days 6 and 9. The growth profile of the control was still in the logarithmic phase on day 12, and the cultures treated with phytohormones reached a plateau on day 9. Phytohormones reduced the aflatoxigenicity of A. parasiticus. The inhibitory activity of the pericarp may be due to the presence of phytohormones.



中文翻译:

小麦果皮如何改变真菌的生长和毒性

减少霉菌毒素造成的经济损失和健康危害,而又不遭受化学杀菌剂的副作用是许多研究人员的目标。当前研究的假设是果皮中天然存在的植物激素可能是果皮对真菌入侵和毒素产生的保护作用。寄生曲霉的孢子悬浮液接种酵母提取物蔗糖(YES)培养基作为对照。使用有或没有YES培养基(PYES或PS)以及含有脱落酸,茉莉酸和赤霉素的YES培养基的果皮水提物来检测这些植物激素对真菌生长和毒素产生的影响。在干重的基础上产生生长曲线,并在研究的每个时间间隔通过HPLC测定黄曲霉毒素的浓度。果皮(PS)的水提物不支持真菌生长或黄曲霉毒素的产生。但是,PYES分别在第3天和第9天显示出最高的真菌生长抑制百分比(51%)和黄曲霉毒素生成抑制百分比(45%)。由于存在植物激素,真菌生长的减少在3天后超过70%,在第12天超过14%;然而,A.寄生虫。果皮的抑制活性可能归因于植物激素的存在。

更新日期:2020-11-12
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