Glutathione peroxidase (GPX) is one of the most important antioxidant enzymes for maintaining reactive oxygen species (ROS) homeostasis. Although studies on fungi have suggested many important physiological functions of GPX, few studies have examined the role of this enzyme in Basidiomycetes, particularly its functions in fruiting body developmental processes. In the present study, GPX-silenced (GPxi) strains were obtained by using RNA interference. The GPxi strains of Hypsizygus marmoreus showed defects in mycelial growth and fruiting body development. In addition, the results indicated essential roles of GPX in controlling ROS homeostasis by regulating intracellular H₂O₂ levels, maintaining GSH/GSSG balance, and promoting antioxidant enzyme activity. Furthermore, lignocellulose enzyme activity levels were reduced and the mitochondrial phenotype and mitochondrial complex activity levels were changed in the H. marmoreus GPxi strains, possibly in response to impediments to mycelial growth and fruiting body development. These findings indicate that ROS homeostasis has a complex influence on growth, fruiting body development, GSH/GSSG balance, and carbon metabolism in H. marmoreus.

Key points

• ROS balance, energy metabolism, fruiting development.

谷胱甘肽过氧化物酶（GPX）是维持活性氧（ROS）稳态的最重要的抗氧化酶之一。尽管对真菌的研究表明GPX具有许多重要的生理功能，但很少有研究检查这种酶在担子菌中的作用，特别是在子实体发育过程中的功能。在本研究中，通过使用RNA干扰获得了GPX沉默（GPxi）菌株。的GPxi株玉蕈显示，菌丝生长和子实体发育缺陷。此外，结果表明GPX通过调节细胞内H ₂ O ₂在控制ROS稳态中的重要作用。保持GSH / GSSG平衡，并促进抗氧化酶活性。此外，木质纤维素酶活性水平降低和线粒体表型和线粒体复合物的活性水平在被改变真姬菇GPxi菌株，可能响应于障碍菌丝生长和子实体发展。这些发现表明ROS稳态对H. marmoreus的生长，子实体发育，GSH / GSSG平衡和碳代谢具有复杂的影响。

关键点

• ROS平衡，能量代谢，果实发育。