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Ni and TiO2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts.
Environmental Science and Pollution Research ( IF 5.8 ) Pub Date : 2020-09-27 , DOI: 10.1007/s11356-020-10908-8
Michal Štefančík 1 , Lucie Válková 1 , Jana Veverková 1 , Jan Balvan 1, 2 , Tomáš Vičar 2 , Petr Babula 2 , Josef Mašek 3 , Pavel Kulich 4 , Monika Pávková Goldbergová 1
Affiliation  

Titanium-based alloys have established a crucial role in implantology. As material deteriorates overtime, nanoparticles of TiO2 and Ni are released. This study is focused on the impact of TiO2 and Ni nanoparticles with size of 100 nm on cytoskeletal and adhesive changes in human physiological and osteoarthritic osteoblasts. The impact of nanoparticles with concentration of 1.5 ng/mL on actin and tubulin expression and gene expression of FAK and ICAM-1 was studied. The cell size and actin expression of physiological osteoblasts decreased in presence of Ni nanoparticles, while TiO2 nanoparticles caused increase in cell size and actin expression. Both cell lines expressed more FAK as a response to TiO2 nanoparticles. ICAM-1 gene was overexpressed in both cell lines as a reaction to both types of nanoparticles. The presented study shows a crucial role of Ni and TiO2 nanoparticles in human osteoblast cytoskeletal and adhesive changes, especially connected with the osteoarthritic cells. Graphical abstract.

中文翻译:

Ni 和 TiO2 纳米颗粒会引起人成骨细胞的粘附和细胞骨架变化。

钛基合金在种植学中已经确立了至关重要的作用。随着材料随着时间的推移而劣化,TiO2 和 Ni 的纳米颗粒被释放出来。本研究的重点是尺寸为 100 nm 的 TiO2 和 Ni 纳米颗粒对人体生理和骨关节炎成骨细胞的细胞骨架和粘附变化的影响。研究了浓度为1.5 ng/mL的纳米粒子对肌动蛋白和微管蛋白表达以及FAK和ICAM-1基因表达的影响。Ni纳米颗粒存在时生理性成骨细胞的细胞大小和肌动蛋白表达降低,而TiO2纳米颗粒导致细胞大小和肌动蛋白表达增加。两种细胞系都表达了更多的 FAK 作为对 TiO2 纳米颗粒的反应。作为对两种类型纳米颗粒的反应,ICAM-1 基因在两种细胞系中都过表达。所提出的研究表明 Ni 和 TiO2 纳米颗粒在人类成骨细胞骨架和粘附变化中的关键作用,特别是与骨关节炎细胞相关。图形概要。
更新日期:2020-09-27
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