This study evaluated an anaerobic fluidized bed reactor to produce hydrogen from sugarcane molasses of 25 g-COD L⁻¹. The reactor of 1.2 L working volume contained shredded tires as support material. The inoculum was sludge obtained in a UASB reactor of a sewage treatment plant. The AFBR was operated at hydraulic retention times of 12, 6, 4 and 3 h. The maximum hydrogen production rate (1.44 L-H₂ h⁻¹ L⁻¹) and the highest hydrogen yield (3.07 mol-H₂ mol⁻¹-glucose) occurred at HRT of 4 and 6 h, respectively. The highest COD removal (23.3 ± 8.5%) was achieved at HRT of 12 h, while the HRT of 6 h presented the maximum carbohydrate conversion of 70.1 ± 2.2%. Ethanol (44–67%) and acetic acid (18–38%) were the main metabolites produced, emphasizing a predominance of ethanol-type fermentation pathway in the process. The PCR-DGGE analysis revealed that the bacterial community presented a maximum similarity of 88% between HRT of 4 and 3 h, indicating that the microbial dynamic altered as the organic load has increased. The highest Shannon-Winner index of 2.77 was obtained at HRT of 6 h, inferring that higher microbial diversity favored hydrogen production.

该研究评估了厌氧流化床反应器从 25 g-COD L ^{-1 的}甘蔗糖蜜生产氢气。1.2L工作容积的反应器含有碎轮胎作为支撑材料。接种物是在污水处理厂的 UASB 反应器中获得的污泥。AFBR 以 12、6、4 和 3 小时的水力停留时间运行。最大产氢率（1.44 LH ₂  h ^-1  L ^-1）和最高产氢率（3.07 mol-H ₂  mol ^-1-葡萄糖）分别发生在 4 和 6 小时的 HRT。最高的 COD 去除率 (23.3 ± 8.5%) 在 12 小时的 HRT 下实现，而 6 小时的 HRT 呈现最大的碳水化合物转化率为 70.1 ± 2.2%。乙醇 (44-67%) 和乙酸 (18-38%) 是产生的主要代谢物，强调了乙醇型发酵途径在该过程中的优势。PCR-DGGE 分析显示细菌群落在 4 和 3 小时的 HRT 之间呈现出 88% 的最大相似性，表明微生物动态随着有机负荷的增加而改变。在 6 小时的 HRT 下获得了最高的香农-温纳指数 2.77，推断较高的微生物多样性有利于产氢。