As the infection by Helicobacter pylori (H. pylori, HP) remains for a lifetime and may induce diseases such as gastric cancer, it is vital to detect and diagnose it. A new non-invasive indirect enzyme-linked immunosorbent assay (iELISA) method based on nano-flowers (NFs) is very advantageous for the sensitive detection of HP. Furthermore, the established iELISA method based on the organic–inorganic bifunctional hybrid nano-flowers including rabbit polyclonal antibody of HP labeled with peroxidase from horseradish (R-HP-Ab–HRP@Cu²⁺ NFs) showed linearity with HP at a concentration of 0–10⁵ CFU mL⁻¹ (R² = 0.9997). Moreover, the limit of detection (LOD) reached 50 CFU mL⁻¹, and not only was the detection sensitivity 20 times higher than that based on rabbit polyclonal antibody of HP labeled with peroxidase from horseradish (R-HP-Ab–HRP) but also the stability of R-HP-Ab–HRP in NFs was improved. In addition, the OD_{450 nm} value was still linearly related to the concentration of HP at a range of 0–10⁵ CFU mL⁻¹ (R² = 0.9952) with a LOD of 50 CFU mL⁻¹ in an artificial saliva system. This study provided a sensitive, low-cost and convenient method for the non-invasive detection of HP.

中文翻译：

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双功能杂种纳米花的制备及其在幽门螺杆菌检测中的应用

由于幽门螺杆菌（H. pylori，HP）的感染会持续一生，并可能诱发胃癌等疾病，因此对其进行检测和诊断至关重要。一种新的基于纳米花（NFs）的非侵入性间接酶联免疫吸附测定（iELISA）方法对于HP的灵敏检测非常有利。此外，基于有机-无机双功能杂种纳米花（包括用辣根过氧化物酶标记的HP兔多克隆抗体（R-HP-Ab–HRP @ Cu ²⁺ NFs）标记的HP的兔多克隆抗体）建立的iELISA方法与HP呈线性关系。 0-10 ⁵ CFU mL ^-1（R ² = 0.9997）。此外，检测限（LOD）达到50 CFU mL^-1，不仅检测灵敏度比辣根过氧化物酶标记的HP兔多克隆抗体（R-HP-Ab-HRP）高20倍，而且R-HP-Ab-HRP在NFs中的稳定性得到了改善。此外，在人工唾液系统中，LOD为50 CFU mL ^-1时，OD _{450 nm}值仍与HP浓度在0-10 ⁵ CFU mL ^-1（ R ² = 0.9952）范围内线性相关。这项研究为非侵入性检测HP提供了一种灵敏，低成本且方便的方法。