Pseudomonas aeruginosa is a common pathogen that is responsible for serious hospital-acquired infections, ventilator-associated pneumonia, and various sepsis syndromes. Also, it is a multidrug-resistant pathogen recognized for its ubiquity and its intrinsically advanced antibiotic-resistant mechanisms. It usually affects immunocompromised individuals but can also infect immunocompetent individuals. There is no vaccine against it available till now. This study predicts an effective epitope-based vaccine against fructose bisphosphate aldolase (FBA) of Pseudomonas aeruginosa using immunoinformatics tools. The protein sequences were obtained from NCBI, and prediction tests were undertaken to analyze possible epitopes for B and T cells. Three B cell epitopes passed the antigenicity, accessibility, and hydrophilicity tests. Six MHC I epitopes were found to be promising, while four MHC II epitopes were found promising from the result set. Nineteen epitopes were shared between MHC I and II results. For the population coverage, the epitopes covered 95.62% worldwide excluding certain MHC II alleles. We recommend in vivo and in vitro studies to prove its effectiveness.

中文翻译：

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使用免疫信息学设计基于表位的针对铜绿假单胞菌果糖二磷酸醛缩酶蛋白的肽疫苗

铜绿假单胞菌是一种常见病原体，可导致严重的医院获得性感染、呼吸机相关性肺炎和各种败血症综合征。此外，它是一种多药耐药病原体，因其普遍存在和内在先进的抗生素耐药机制而得到认可。它通常影响免疫功能低下的个体，但也可以感染免疫功能正常的个体。目前还没有针对它的疫苗。这项研究预测了一种针对铜绿假单胞菌果糖二磷酸醛缩酶 (FBA) 的有效表位疫苗使用免疫信息学工具。蛋白质序列是从 NCBI 获得的，并进行了预测测试以分析 B 和 T 细胞的可能表位。三个 B 细胞表位通过了抗原性、可及性和亲水性测试。从结果集中发现六个 MHC I 表位是有希望的，而四个 MHC II 表位被发现有希望。MHC I 和 II 结果之间共有 19 个表位。在人群覆盖率方面，表位覆盖了全球95.62%，不包括某些MHC II等位基因。我们建议进行体内和体外研究以证明其有效性。