ABSTRACT

This study represents the green synthesis of silver nanoparticles (AgNPs) using Ipomoea aquatica (Water Spinach) leaf extract, and investigate the cytotoxicity and antimicrobial activities. The Ipomoea aquatica (IA) mediated green synthesized AgNPs (IA-AgNPs) was characterized by ultraviolet–visible (UV–VIS.) spectrophotometry, Fourier transform-infrared (FT-IR) spectroscopy, atomic absorption spectrometry (AAS), X-ray deffractometry (XRD), transmission electron microscopy (TEM), and energy dispersive X-ray (EDX) spectroscopy. The results showed that the material was formed in nano-dimension with particle size in the range between 15–30 nm. The nanoparticles (NPs) were almost spherical in shape and demonstrated excellent material stability. The PC12 cell model in-vitro was used to determine the cytotoxicity of the IA-AgNPs, and for antimicrobial study, E. coli, Salmonella, and Staphylococcus sp. were employed. The cell viability results showed that the NPs did not induce any cytotoxicity until 10 ppm for 48 h of incubation, and it was further confirmed by the lactate dehydrogenase (LDH) assay. Moreover, IA-AgNPs demonstrated good antibacterial activity against three bacteria: E. coli, Salmonella, and Staphylococcus sp. Thus, this study may create an important synthetic route for the production of green-AgNPs and their safe usages, and may also be used to augment the bioactivities of the material with minimum toxicity.

摘要

这项研究代表了使用水生番薯叶提取物绿色合成银纳米颗粒（AgNPs）的方法，并研究了其细胞毒性和抗菌活性。的蕹（IA）介导的绿色合成的AgNPs（IA-的AgNPs）分光光度法表征利用紫外可见（UV-VIS），傅里叶变换红外（FT-IR）光谱法，原子吸收光谱（AAS），X射线折光法（XRD），透射电子显微镜（TEM）和能量色散X射线（EDX）光谱。结果表明，该材料以纳米尺寸形成，粒径在15–30 nm之间。纳米粒子（NPs）的形状几乎为球形，并具有出色的材料稳定性。体外PC12细胞模型用于确定IA-AgNPs的细胞毒性，并用于大肠杆菌，沙门氏菌和葡萄球菌sp。的抗菌研究。被雇用。细胞活力结果表明，NPs直到48小时培养10 ppm才诱导细胞毒性，并且通过乳酸脱氢酶（LDH）分析进一步证实。此外，IA-AgNPs对三种细菌表现出良好的抗菌活性：大肠杆菌，沙门氏菌和葡萄球菌。因此，这项研究可能为绿色AgNPs的生产及其安全使用创造一条重要的合成途径，也可以用于以最小的毒性来增强该材料的生物活性。