ABSTRACT Mentha longifolia . is a perennial herb of Mentha in Labiatae. Considering the biological activities of Mentha longifolia essential oils, this work was planned to study the yields, relative percentage content and semi-quantitative change of chemical constituents as well as the biological activities of essential oils extracted by different extraction methods. Steam-distillation (SD), lipophilic solvents (n-hexane) extraction (LSE) and supercritical CO2 fluid extraction (SC-CO2) were employed to produce essential oils from Mentha longifolia. The essential oil obtained from LSE (1.21 ± 0.06%, w/w) showed the highest yield. A total of 39 compounds were identified by gas chromatography/flame ionization detector (GC-FID) and gas chromatography/mass spectrometry (GC-MS). The major compounds in SD and LSE were carvone, limonene, trans-caryophyllene and α-Terpineol, the major compounds in SC-CO2 were carvone, trans-caryophyllene, trans-β-Farnesene and Germacrene D. All essential oils showed varying degrees of antioxidant and anti-COX-2 activity. The IC50 ranging from (0.69 ± 0.01 ~ 15.61 ± 0.16 mg/mL) for DPPH and (0.16 ± 0.001 ~ 2.19 ± 0.11 mg/mL) for ABTS, SC-CO2 produced essential oil showed the highest scavenging activity both on DPPH and ABTS. Meanwhile, all the EOs showed the strong inhibition activity on COX-2, the EO obtained by SC-CO2 also showed the highest capacity to inhibit COX-2.

中文翻译：

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长叶薄荷精油的化学成分和生物活性：不同提取方法的影响

摘要长叶薄荷。是唇形科薄荷属的多年生草本。考虑到长叶薄荷精油的生物活性，本工作拟研究不同提取方法提取的精油的产量、相对百分比含量和化学成分的半定量变化以及生物活性。采用蒸汽蒸馏 (SD)、亲脂溶剂 (正己烷) 萃取 (LSE) 和超临界 CO2 流体萃取 (SC-CO2) 从长叶薄荷中生产精油。从 LSE 获得的精油 (1.21 ± 0.06%, w/w) 的产量最高。通过气相色谱/火焰离子化检测器 (GC-FID) 和气相色谱/质谱 (GC-MS) 共鉴定了 39 种化合物。SD 和 LSE 中的主要化合物是香芹酮、柠檬烯、反式石竹烯和α-松油醇，SC-CO2 中的主要化合物是香芹酮、反式石竹烯、反式-β-法呢烯和 Germacrene D。所有精油都表现出不同程度的抗氧化和抗 COX-2 活性。DPPH 的 IC50 范围为 (0.69 ± 0.01 ~ 15.61 ± 0.16 mg/mL) 和 ABTS 的 (0.16 ± 0.001 ~ 2.19 ± 0.11 mg/mL)，SC-CO2 产生的精油对 DPPH 和 ABTS 的清除活性最高. 同时，所有的EOs都对COX-2表现出很强的抑制活性，SC-CO2得到的EO也表现出最高的抑制COX-2的能力。001 ~ 2.19 ± 0.11 mg/mL) 对于 ABTS，SC-CO2 产生的精油对 DPPH 和 ABTS 均显示出最高的清除活性。同时，所有的EOs都对COX-2表现出很强的抑制活性，SC-CO2得到的EO也表现出最高的抑制COX-2的能力。001 ~ 2.19 ± 0.11 mg/mL) 对于 ABTS，SC-CO2 产生的精油对 DPPH 和 ABTS 均显示出最高的清除活性。同时，所有的EOs都对COX-2表现出很强的抑制活性，SC-CO2得到的EO也表现出最高的抑制COX-2的能力。