The diversity of vegetative compatibility (vc) types of chestnut blight fungus, Cryphonectria parasitica, has been reported to be very low in all of Turkey, except in the eastern Black Sea Region. However, risk for increase in vc type diversity has been pointed out regarding the emergence of new vc types as recombinants during sexual reproduction. In this study, the potential increase in the diversity of vc types in Turkish populations of C. parasitica was demonstrated by experimental mating crosses between the two dominant vc types, EU‐1 and EU‐12. Two local isolates of C. parasitica, K5‐5 (EU‐1) and A‐2490 (EU‐12), were used setting crosses on autoclaved chestnut wood. Among the offspring from the crosses, two parental (EU‐1 and EU‐12) and 14 non‐parental vc types (EU‐2, EU‐3, EU‐4, EU‐7, EU‐8, EU‐11, EU‐14, EU‐17, EU‐22, EU‐25, EU‐26, EU‐28, EU‐29 and EU‐30) were detected by a routine test based on co‐culturing. Sixteen vc genotypes were confirmed by a PCR assay using specific primers targeting the alleles of six vic loci in C. parasitica. In field studies, no evidence of new vc types as recombinants was found in western Turkey whereas 14 different vc types were detected among single ascospore isolates isolated from field‐collected perithecia, which were sampled from the eastern Black Sea region. The results of this study indicate that the potential increase in vc type diversity in Turkish population of C. parasitica is high under the conditions where C. parasitica reproduces sexually.

中文翻译：

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土耳其寄生ry种群营养相容性类型的潜在多样性

营养兼容性（VC）类型栗疫病菌的多样性板栗疫病，据报道，在所有土耳其非常低，除了在东部黑海地区。然而，关于性繁殖期间新的vc类型作为重组体的出现，已经指出了vc类型多样性增加的风险。在这项研究中，通过两种主要的vc类型EU-1和EU-12之间的实验交配，证明了土耳其寄生虫C. vc类型多样性的潜在增加。寄生寄生梭状芽胞杆菌的两个局部分离株，K5-5（EU-1）和A-2490（EU-12）用于在高压灭菌的栗木上打叉。在十字架的后代中，有两种亲本（EU-1和EU-12）和14种非亲本vc类型（EU-2，EU-3，EU-4，EU-7，EU-8，EU-11，通过基于共培养的常规测试可以检测到EU-14，EU-17，EU-22，EU-25，EU26，EU28，EU29和EU-30。通过PCR测定证实16种vc基因型，使用特异性引物靶向寄生衣原体中6个vic位点的等位基因。。在野外研究中，在土耳其西部没有发现新的vc类型的重组体证据，而在从黑海东部采样的田间皮膜分离的单个子囊分离物中分离到14种不同的vc类型。这项研究的结果表明，在寄生寄生衣藻有性繁殖的条件下，土耳其寄生寄生衣藻的vc类型多样性的潜在增加很高。