In 2015 and 2016, lettuce (Lactuca sativa) plants showing yellow ringspots and line patterns were observed in Argolida (Peloponnese, Greece). Symptoms resembled those caused by nepoviruses, especially artichoke yellow ringspot virus (AYRSV), an endemic virus in artichoke (Cynara scolymus) and onion (Allium cepa var. cepa) crops in this area. The aim of this work was to identify and characterize the viral agent associated with the observed disease. We carried out mechanical inoculations onto Nicotiana benthamiana and lettuce plants and also virus‐specific reverse transcription (RT)‐PCR. Results confirmed the presence of AYRSV in all lettuce plants with symptoms tested, and the natural and experimental host range of the virus was studied. The species Heliotropium europaeum, Solanum nigrum, Origanum vulgare, Lactuca seriola, and Sinapis arvensis, are recorded as hosts of AYRSV for the first time. Also, AYRSV was detected by RT‐PCR in seeds and in the produced seedlings of lettuce, N. benthamiana, N. glutinosa, N. tabacum, and S. nigrum. Sequencing of a partial fragment of the gene encoding the RNA‐dependent RNA polymerase was performed for 29 isolates of AYRSV originating from different hosts (lettuce, artichoke, onion, and arable weeds). Phylogenetic analysis of AYRSV isolates classified them in diverse clusters that exhibited no host grouping. All groups included isolates from cultivated and wild plant species. In this study, AYRSV was associated with a new lettuce disease for the first time, thus increasing the known natural host range of the virus.

中文翻译：

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朝鲜蓟黄环斑病毒是一种新的莴苣病毒性疾病的病原体：流行病学和分子变异性

在2015年和2016年，在Argolida（伯罗奔尼撒，希腊）观察到​​了具有黄色环斑和线型的莴苣（莴苣）植物。症状类似于那些由nepoviruses，尤其是朝鲜蓟黄环斑病毒（AYRSV），在菊芋的流行病毒（朝鲜蓟），洋葱（洋葱变种CEPA）作物在这一领域。这项工作的目的是鉴定和表征与观察到的疾病有关的病毒制剂。我们对本氏烟草进行了机械接种和生菜植物，以及病毒特异性逆转录（RT）-PCR。结果证实了所有莴苣植物中存在AYRSV，并对其症状进行了测试，并对该病毒的天然和实验宿主范围进行了研究。种天芥菜，龙葵，牛至，莴苣鰤，和白芥，被记录为首次AYRSV的主机。另外，AYRSV通过RT-PCR在种子和生菜，所生产的幼苗检测本塞姆氏烟草，N.黄，普通烟草，和龙葵。对源自不同宿主（生菜，朝鲜蓟，洋葱和可耕杂草）的29种AYRSV分离株进行了编码RNA依赖性RNA聚合酶基因的部分片段测序。AYRSV分离株的系统发生分析将它们分类为没有主机分组的不同簇。所有组均包括来自栽培和野生植物物种的分离株。在这项研究中，AYRSV首次与一种新的莴苣疾病相关，因此增加了已知的病毒自然宿主范围。