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Increased substrate availability reveals the potential of scentless lisianthus flowers in producing fragrant benzenoid‐phenylpropanoids
Physiologia Plantarum ( IF 6.4 ) Pub Date : 2020-12-27 , DOI: 10.1111/ppl.13264
Fang Fang 1 , Moran Oliva 1, 2 , Rinat Ovadia 1 , Einat Bar 3 , Ada Nissim‐Levi 1 , Varun Kumar 1 , Ru Wang 1 , Agmon Neeman 1 , Michele Zaccai 4 , Efraim Lewinsohn 3 , Michal Oren‐Shamir 1
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Lisianthus (Eustoma grandiflorum), a leading plant in the cut flower industry is scentless. Here we show that lisianthus flowers have potential to produce several fragrant benzenoid-phenylpropanoids when substrate availability is not limited. To enable hyperaccumulation of substrates for the production of volatile benzenoid-phenylpropanoids, lisianthus commercial hybrid 'Excalibur Pink' was transformed via floral dipping with a feedback-insensitive E.coli DAHP synthase (AroG*) and Clarkia breweri benzyl alcohol acetyltransferase (BEAT), under constitutive promoters. The T1 progeny of 'Excalibur Pink' plants segregated into four visual phenotypes, with pink or white colored petals and multiple or single petal layers. Interestingly, transformation with AroG* and BEAT caused no significant effect in the pigment composition among phenotypes, but did increase the levels of down-stream fragrant volatile benzenoids. All the transgenic lines exclusively accumulated methyl benzoate, a fragrant benzenoid, either in their petals or leaves. Furthermore, feeding with benzyl alcohol, resulted in the accumulation of two novel benzenoids, benzyl acetate (the product of BEAT) and benzoate, as well as a dramatic increase in the concentrations of additional benzenoid-phenylpropanoid volatiles. Presumably, the degree of benzaldehyde overproduction after benzyl alcohol feeding in both leaves and flowers revealed their reverse conversion in lisianthus plants. These findings demonstrate the concealed capability of lisianthus plants to produce a wide array of fragrant benzenoid-phenylpropanoids, given high substrate concentrations, which could in turn open opportunities for future scent engineering. This article is protected by copyright. All rights reserved.

中文翻译:

底物可用性的增加揭示了无味桔梗花在生产芳香苯类-苯丙烷方面的潜力

洋桔梗(Eustoma grandiflorum)是切花行业的领先植物,是无味的。在这里,我们表明,当底物可用性不受限制时,桔梗花有可能产生几种芳香的苯类-苯丙烷。为了能够产生挥发性苯类-苯丙烷的底物超积累,通过使用反馈不敏感的大肠杆菌 DAHP 合酶 (AroG*) 和 Clarkia breweri 苯甲醇乙酰转移酶 (BEAT) 进行花卉浸渍,转化了洋桔梗商业杂交种“Excalibur Pink”,在组成型启动子下。'Excalibur Pink' 植物的 T1 后代分为四种视觉表型,具有粉红色或白色花瓣以及多个或单个花瓣层。有趣的是,用 AroG* 和 BEAT 进行转化对表型中的色素组成没有显着影响,但确实增加了下游芳香挥发性苯类的水平。所有转基因品系都在花瓣或叶子中专门积累苯甲酸甲酯,一种芳香的苯类。此外,喂食苯甲醇会导致两种新型苯类物质的积累,即乙酸苄酯(BEAT 的产物)和苯甲酸盐,以及额外苯类-苯丙烷挥发物的浓度急剧增加。据推测,叶和花中苯甲醇喂养后苯甲醛过量产生的程度表明它们在桔梗植物中的反向转化。这些发现证明了桔梗植物产生多种芳香苯类-苯丙烷的隐藏能力,鉴于高底物浓度,这反过来可能为未来的气味工程打开机会。本文受版权保护。版权所有。
更新日期:2020-12-27
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