Paramoeba perurans causes amoebic gill disease (AGD), which is a major problem in aquaculture worldwide. The parasite can be cultured in vitro, but to this date, no method for long‐term storage of the clones exists. In this study, we describe a method for cryopreservation of Paramoeba perurans. The method was successfully employed on four out the five clones we tested. The thawing success rate, that is the percentage of successfully thawed vials relative to the total number of vials that were thawed, differed for the clones and ranged from 25% to 100%. The age of the clones seemed to have a negative impact on the ability to survive cryopreservation.

中文翻译：

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冻存巴拉莫巴的方法

百草枯杆菌引起阿米巴g病（AGD），这是全世界水产养殖的主要问题。可以在体外培养该寄生虫，但是到目前为止，还没有用于克隆长期保存的方法。在这项研究中，我们描述了一种冻存Peramoeba perurans的方法。该方法已成功应用于我们测试的五个克隆中的四个。克隆的解冻成功率（即成功解冻的小瓶相对于解冻的小瓶总数的百分比）有所不同，范围从25％到100％。克隆的年龄似乎对冷冻保存的生存能力有负面影响。