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Addition of glycerol enhances the flexibility of gelatin hydrogel sheets; application for in utero tissue engineering
Journal of Biomedical Materials Research Part B: Applied Biomaterials ( IF 3.4 ) Pub Date : 2020-11-09 , DOI: 10.1002/jbm.b.34756
Miho Watanabe 1, 2 , Haiying Li 1 , Masaya Yamamoto 3, 4 , Jun-Ichi Horinaka 5 , Yasuhiko Tabata 3 , Alan W Flake 1
Affiliation  

Gelatin hydrogels are naturally derived scaffolds useful for tissue engineering because of their cytocompatibility and controllable degradability. However, they are brittle and inflexible when dry, which limits their use for in utero tissue engineering in large animal models. Therefore, in this study, we attempted to generate flexible gelatin sheets by adding various plasticizers with different molecular weights (MW). We systematically evaluated the flexibility, sustainability, and potential clinical utility of the resulting flexible gelatin sheets. Gelatin sheets with low‐MW plasticizers, such as monosaccharides or sugar alcohols, showed a reduced tensile modulus in dynamic viscoelasticity, which reflected their actual flexibility. Wet gelatin sheets containing plasticizers showed higher tensile strength than the nonplasticizer control, although wet gelatin sheets under all conditions had a much lower tensile strength than dry gelatin sheets. In a functional study, gelatin sheets containing glycerol, which has the lowest MW among sugar alcohols, showed encouraging results, such as good fit to the curvature of the experimental animal, biocompatibility, and suitability for endoscopic approaches. The findings of this study should enable the expansion of future applications for flexible gelatin sheets.

中文翻译:

添加甘油可增强明胶水凝胶片材的柔韧性;子宫内组织工程应用

明胶水凝胶是天然衍生的支架,可用于组织工程,因为它们具有细胞相容性和可控降解性。然而,它们在干燥时易碎且不灵活,这限制了它们在大型动物模型中的子宫内组织工程中的使用。因此,在本研究中,我们尝试通过添加不同分子量 (MW) 的各种增塑剂来生产柔性明胶片。我们系统地评估了所得柔性明胶片的灵活性、可持续性和潜在的临床效用。含有低分子量增塑剂(如单糖或糖醇)的明胶片在动态粘弹性中表现出降低的拉伸模量,这反映了它们的实际柔韧性。含有增塑剂的湿明胶片显示出比不含增塑剂的对照更高的拉伸强度,尽管在所有条件下湿明胶片的拉伸强度都比干明胶片低得多。在一项功能性研究中,含有甘油的明胶片(在糖醇中分子量最低)显示出令人鼓舞的结果,例如与实验动物的曲率有良好的贴合性、生物相容性以及内窥镜方法的适用性。这项研究的结果应该能够扩展柔性明胶片的未来应用。
更新日期:2020-11-09
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