Saccharomyces cerevisiae is an important model to study the molecular mechanisms of eukaryotic cell aging. In previous studies, the replicative lifespan (RLS) of yeast is analyzed mainly by manual microdissection. Microfluidic technologies have gradually replaced this labor‐intensive operation. Many microfluidic devices can trap a single yeast cell and track its entire lifespan. However, a direct comparison between microfluidic device designs and the corresponding evaluated RLS values is limited by the different experimental conditions. This study establishes a microfluidic platform for a systematic comparison among different cell trapping structures. The platform integrates four representative structures (micropads, microchannels, three‐bar jails, and two‐bar cups) and allows simultaneous control with a uniform external condition. The experimentally evaluated RLS values are affected by specific chip structures. Exploration of the underlying mechanism revealed that external mechanical stress caused by the chip structure is the key factor in reducing RLS. The findings indicate that external mechanical stress should be a major reference factor in the selection of microfluidic devices. The study also highlights the importance of further research on the relationship between mechanical stress and aging.

中文翻译：

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集成微流控系统在不同诱捕结构中酵母复制寿命的比较分析

酿酒酵母是研究真核细胞衰老的分子机制的重要模型。在以前的研究中，酵母的复制寿命（RLS）主要通过手动显微解剖进行分析。微流体技术已逐渐取代了这种劳动强度大的操作。许多微流体装置可以捕获单个酵母细胞并跟踪其整个寿命。但是，微流体装置设计与相应评估的RLS值之间的直接比较受到不同实验条件的限制。这项研究建立了一个微流体平台，用于系统比较不同的细胞捕获结构。该平台集成了四个代表性结构（微垫，微通道，三杆监狱和两杆杯），并允许在统一的外部条件下进行同时控制。实验评估的RLS值受特定芯片结构的影响。对潜在机理的探索表明，由芯片结构引起的外部机械应力是降低RLS的关键因素。研究结果表明，外部机械应力应成为选择微流控设备的主要参考因素。该研究还强调了进一步研究机械应力和老化之间关系的重要性。