Background

Mucor circinelloides is an opportunistic fungus capable of causing mucormycosis, a highly aggressive infection of quick spreading. Besides, it also has a high mortality rate due to late diagnosis and difficult treatment.

Aims

In this study we have identified the most immunoreactive proteins of the secretome and the total protein extract of M. circinelloides using sera from immunocompromised infected mice.

Methods

The proteins of the secretome and the total extract were analyzed by two-dimensional electrophoresis and the most immunoreactive antigens were detected by Western Blot, facing the sera of immunocompromised infected mice to the proteins obtained in both extracts of M. circinelloides.

Results

Seven antigens were detected in the secretome extract, and two in the total extract, all of them corresponding only to three proteins. The enzyme enolase was detected in both extracts, while triosephosphate isomerase was detected in the secretome, and heat shock protein HSS1 in the total extract.

Conclusions

In this work the most immunoreactive antigens of the secretome and the total extract of M. circinelloides were identified. The identified proteins are well known fungal antigens and, therefore, these findings can be useful for future research into alternatives for the diagnosis and treatment of mucormycosis.

中文翻译：

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免疫功能低下感染小鼠血清识别的圆环毛霉抗原的鉴定

背景

Mucor circinelloides是一种机会性真菌，能够引起毛霉菌病，这是一种快速传播的高度侵袭性感染。此外，由于诊断晚，治疗困难，死亡率也很高。

宗旨

在这项研究中，我们已经确定的分泌物和总蛋白提取物的免疫反应最蛋白质M.卷枝利用受感染的免疫功能低下小鼠的血清。

方法

分泌蛋白质和蛋白质的总提取物通过双向电泳分析，并通过Western印迹法检测最免疫反应的抗原，免疫功能低下面临感染小鼠到的两种提取物得到的蛋白的血清M.卷枝。

结果

在分泌组提取物中检测到七种抗原，在总提取物中检测到两种抗原，所有这些抗原都仅对应于三种蛋白质。在两种提取物中均检测到烯醇化酶，而在分泌组中检测到丙糖磷酸异构酶，在总提取物中检测到热休克蛋白 HSS1。

结论

在这项工作中的分泌物和总提取物的最免疫抗原M.卷枝进行鉴定。鉴定出的蛋白质是众所周知的真菌抗原，因此，这些发现可用于未来研究毛霉菌病诊断和治疗的替代方案。