The construction of large microbial fuel cells (MFCs) and their long-term reliability are current challenges. MFCs generate power while purifying wastewater, save electricity, avoid pollutant stripping into the air and are a source of CO₂. To understand larger MFCs, a 1000-L MFC was designed. It was built from transparent polyester and electrodes were from reticulated vitreous carbon. Four power management devices were connected to an ensemble of 64 MFC units and assembled as a 12 m long MFC. Two Raspberry and a personal computer with Python programmed software automatized power management. The MFC was run for one year under maximum power point tracking (MPPT). Temperatures between 11.5 °C and 21 °C corresponded to WWTP conditions. The reactor shared electrolytes within 12 m long half-cells and 80–95% COD was removed generating 0.015 to 0.060 kWh/m³ with an energy efficiency of 5.8–12.1%. Voltage reversal were seen as potential imbalances among MFC units and all self-healing. Ammonium removal reached 48%, phosphorous was reduced to 0.59 mg/L, and micropollutants degraded by 67%. Biofilm mapping by 16S rRNA metagenomics indicated bi-sectorial metabolic properties. 10 Major genera were essential in the elongated scale up MFC generating electricity, reduced energy needed, and purified wastewater.

大型微生物燃料电池（MFCs）的结构及其长期可靠性是当前的挑战。MFC在净化废水的同时发电，节省电力，避免污染物排放到空气中，并且是CO ₂的来源。为了理解较大的MFC，设计了1000升MFC。它由透明聚酯制成，电极由网状玻璃碳制成。四个电源管理设备连接到64个MFC单元的集合，并组装成12 m长的MFC。两台Raspberry和一台装有Python编程软件的个人计算机可自动进行电源管理。MFC在最大功率点跟踪（MPPT）下运行了一年。11.5°C和21°C之间的温度对应于WWTP条件。该反应堆在12 m长的半电池内共享电解质，去除了80–95％的COD，产生0.015至0.060 kWh / m ³能源效率为5.8-12.1％。电压反转被视为MFC单元之间的潜在失衡和所有自我修复。铵去除率达到48％，磷减少至0.59 mg / L，微污染物降解了67％。通过16S rRNA宏基因组学进行生物膜作图表明了双部门代谢特性。10大类对于延长MFC发电，减少所需能源和净化废水至关重要。