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Value-added switchgrass extractives for reduction of Escherichia coli O157:H7 and Salmonella Typhimurium populations on Formica coupons
Food Microbiology ( IF 5.3 ) Pub Date : 2020-11-07 , DOI: 10.1016/j.fm.2020.103674
J.M. Choi , E. Camfield , A. Bowman , K. Rajan , N. Labbé , K.D. Gwinn , B.H. Ownley , N. Moustaid-Moussa , D.H. D'Souza

Recurring outbreaks linked to Escherichia coli O157:H7-contaminated lettuce and Salmonella enterica-contaminated sprouts highlight the need for improved food safety measures. The aim of this study was to determine the ability of a bio-based antimicrobial extract prepared from switchgrass, a dedicated energy crop, to decrease E. coli O157:H7 and S. Typhimurium populations on Formica coupons, a model food-contact surface. Overnight cultures of ∼7 log CFU/mL E. coli O157:H7 and S. Typhimurium, air-dried on Formica coupons were treated with 0.625% NaClO, 70% ethanol, sterile water or different batches of switchgrass extractives (SE1, SE2, and SE3) for up to 30 min. SE3 reduced E. coli O157:H7 by 4.43 log CFU/mL after 1 min and to non-detectable levels after 1 min by all other treatments. Populations of S. Typhimurium LT2 (15-min drying) were reduced by 3.30 log CFU/mL with 70% ethanol, 5.38 log CFU/mL with SE1, and to non-detectable levels with 0.625% NaClO after 1 min, while S. Typhimurium ATCC 23564 (1-h drying) was non-detectable after 1 min by all treatments. Under soiled conditions, 10-min treatment with SE1 and 70% ethanol reduced both bacteria to non-detectable levels. Studies with concentrated switchgrass extractives combined with other natural disinfectants or in hurdle approaches warrant further investigation.



中文翻译:

增值柳枝extract提取物,用于减少福米卡优惠券上的大肠杆菌O157:H7和鼠伤寒沙门氏菌种群

大肠杆菌O157:H7污染的生菜和肠炎沙门氏菌污染的芽苗有关的反复暴发凸显了改进食品安全措施的必要性。这项研究的目的是确定从柳枝,(一种专用的能源作物)制备的生物基抗菌素提取物降低E的能力大肠杆菌O157:H7和S.上福米卡券,模型食品接触表面鼠伤寒群。过夜培养约7 log CFU / mL大肠杆菌O157:H7和S。在Formica试片上风干的鼠伤寒用0.625%NaClO,70%乙醇,无菌水或不同批次的柳枝提取物(SE1,SE2和SE3)处理长达30分钟。SE3在1分钟后将大肠杆菌O157:H7降低4.43 log CFU / mL,并在所有其他处理后1分钟后降低到不可检测的水平。群S.鼠伤寒沙门氏菌LT2(15分钟干燥),通过3.30日志CFU / mL的减少用70%乙醇,5.38日志CFU /毫升SE1,和非可检测的水平与0.625%的NaClO 1分钟后,而S.所有处理均在1分钟后未检测到鼠伤寒ATCC 23564(干燥1小时)。在脏污的条件下,用SE1和70%乙醇处理10分钟可将两种细菌均降至无法检测的水平。浓缩柳枝extract提取物与其他天然消毒剂结合或采用障碍法进行的研究值得进一步研究。

更新日期:2020-11-09
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