Clove (Eugenia caryophyllata Thunb.) essential oil (CEO) has been extensively utilized and well-recognized due to its wide medicinal activities. In our study, the gas chromatography–mass spectrometry (GC–MS) technology was applied in characterizing the CEO chemical composition. Altogether 58 volatile components were discovered from bud CEO. To further clarify the antioxidant and anti-aging activities of CEO in vivo, the nematode C. elegans was used as an animal model. The results suggested that, chronic CEO treatment significantly extended the lifespan and promoted the production and health. As expected, CEO possessed antioxidant activities against the reactive oxygen species (ROS) through inducing the expression of SOD-3 or GST-4. Meanwhile, it was found that CEO treatment induced the DAF-16/FOXO nuclear translocation from the cytoplasm. Finally, the results indicated that CEO caused the apoptosis of germ cells in acep-1 and daf-16 dependent manner. Overall, our research suggests that CEO possesses antioxidant and anti-aging activities, and that DAF-16 may be essential for its biological functions.

丁香（Eugenia caryophyllata Thunb。）精油（CEO）由于其广泛的医学活性而被广泛使用和公认。在我们的研究中，气相色谱-质谱（GC-MS）技术用于表征CEO化学成分。芽首席执行官总共发现了58种挥发性成分。为了进一步阐明首席执行官体内线虫秀丽隐杆线虫的抗氧化和抗衰老活性被用作动物模型。结果表明，长期的CEO治疗显着延长了寿命，促进了生产和健康。正如预期的那样，CEO通过诱导SOD-3或GST-4的表达而具有针对活性氧（ROS）的抗氧化活性。同时，发现CEO治疗诱导了DAF-16 / FOXO从细胞质的核易位。最后，结果表明CEO以cep-1和daf-16依赖性方式引起生殖细胞凋亡。总体而言，我们的研究表明CEO具有抗氧化和抗衰老的功能，DAF-16可能对其生物学功能至关重要。