This work presents the development of disposable biosensors used in the determination of biogenic amines. The biosensors were fabricated using diamine oxidase (DAOx) or monoamine oxidase (MAOx) enzyme- and platinum nanoparticle (PtNP)-modified screen-printed carbon electrodes (SPCE). The morphological and electrochemical properties of the biosensors were examined by scanning electron microscopy, energy-dispersive X-ray spectroscopy, electrochemical impedance spectroscopy, and cyclic voltammetry measurements. Amperometric measurements indicated that the DAOx/PtNP/SPCE biosensor responded to histamine, putrescine, cadaverine, spermine, spermidine, β-phenylethylamine, tryptamine, and tyramine; however, its MAOx-based counterpart showed no response towards putrescine and cadaverine. A performance comparison of two biosensors indicated that the one based on DAOx had a linear concentration range from 5.3 × 10^− 7 to 7.2 × 10^− 5 M and the other based on MAOx from 3.9 × 10^− 7 to 7.6 × 10^− 5 M for tyramine. The sensitivities of the DAOx- and MAOx-based biosensors towards tyramine were 24.8 µA mM^− 1 and 46.1 µA mM^− 1, respectively. The proposed biosensors were tested for analysis of blue cheese sample spiked with known concentration of tyramine for the verification of biosensor applicability for real samples.

Graphic abstract

中文翻译：

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基于铂纳米粒子修饰的丝网印刷碳电极的一次性生物传感器，用于测定生物胺

这项工作介绍了用于确定生物胺的一次性生物传感器的发展。生物传感器是使用二胺氧化酶（DAOx）或单胺氧化酶（MAOx）酶和铂纳米颗粒（PtNP）修饰的丝网印刷碳电极（SPCE）制成的。通过扫描电子显微镜，能量色散X射线光谱，电化学阻抗光谱和循环伏安法测量，检查了生物传感器的形态和电化学特性。安培测量表明DAOx / PtNP / SPCE生物传感器对组胺，腐胺，尸胺，亚精胺，亚精胺，β响应-苯乙胺，色胺和酪胺；但是，其基于MAOx的对应物对腐胺和尸胺没有反应。2个生物传感器的性能比较表明，基于DAOx所述一个具有线性浓度范围从5.3×10 ^{- 7}至7.2×10 ^{- 5}  M和其他基于MAOx从3.9×10 ^{- 7}至7.6×10 ^{- 5} 中号酪胺。基于DAOx和MAOx的生物传感器对酪胺的敏感性分别为24.8 µA mM ^-1和46.1 µA mM ^-1。测试了建议的生物传感器，以分析掺有已知浓度酪胺的蓝纹奶酪样品，以验证生物传感器对实际样品的适用性。

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