Abstract

Euphorbia hirta and Euphorbia thymifolia are plants with the same regional name “Dudheli” in Gujarat region, India. The present study has been conducted with comparative analysis of two plants, E. hirta and E. thymifolia, for total flavonoid content, total phenolic content, and quantification of biomarkers (rutin, gallic acid, and quercetin) by using high-performance thin-layer chromatography (HPTLC). Total phenolic contents (TPC) and total flavonoid contents (TFC) were evaluated by Folin‒Ciocalteu method and aluminum chloride colorimetric method, respectively. An improved HPTLC method was developed and validated for the simultaneous estimation of rutin, quercetin, and gallic acid. TPC was found more in the methanolic extract of E. hirta, and TFC was found almost the same in ethyl acetate extracts of both plants. Both plants showed the presence of rutin, quercetin, and gallic acid. The results of the HPTLC study provided a valuable bioactive marker content comparison between two species of the Euphorbiaceae family.

Graphic abstract

中文翻译：

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两种大戟科植物Dugdhika的比较定量植物化学和HPTLC分析

摘要

Euphorbia hirta和Euphorbia thymifolia是印度古吉拉特邦地区名称相同的植物“ Dudheli”。本研究是通过使用高效能薄层色谱法对两种植物（E. hirta和E. thymifolia）的总黄酮含量，总酚含量和生物标志物（芦丁，没食子酸和槲皮素）的定量分析进行比较分析。层色谱法（HPTLC）。总酚含量（TPC）和总黄酮含量（TFC）分别通过Folin‒Ciocalteu法和氯化铝比色法进行评估。开发了一种改进的HPTLC方法，并经过验证可同时评估芦丁，槲皮素和没食子酸。TPC的甲醇提取物中发现更多在两种植物的乙酸乙酯提取物中发现大肠埃希菌和TFC几乎相同。两种植物均显示出芦丁，槲皮素和没食子酸的存在。HPTLC研究的结果提供了大戟科两个物种之间有价值的生物活性标记物含量比较。

图形摘要