Peanut is widely grown and provides protein and edible oil for millions of people. Peanut growth and productivity are frequently negatively affected by abiotic and biotic environmental factors. However, the research on improving peanut germplasm resources by genetic transformation is very limited. Here, the novel R2R3-MYB repressor GmMYB3a was introduced into peanut plants by Agrobacterium-mediated transformation for the first time for thorough evaluation of the function of GmMYB3a in drought stress plant responses. We generated GmMYB3a-transgenic peanut plants. The GmMYB3a-overexpressing lines showed significantly improved physiological responses and no yield loss non-transgenic plants, in terms of survival rates. Thus, the GmMYB3a-overexpressing plants showed better photosynthetic performance, higher relative water content, and greater water use efficiency, demonstrating their adaptive capacity to water deficit. We conclude that overexpression of GmMYB3a can improve drought tolerance and productivity in peanut.

花生被广泛种植，为数百万人提供蛋白质和食用油。花生的生长和生产力经常受到非生物和生物环境因素的负面影响。然而，通过遗传转化改良花生种质资源的研究非常有限。在这里，首次通过农杆菌介导的转化将新型 R2R3-MYB 阻遏物GmMYB3a引入花生植物，以彻底评估GmMYB3a在干旱胁迫植物响应中的功能。我们生成了GmMYB3a -转基因花生植物。该GmMYB3a- 就存活率而言，过表达株系显示出显着改善的生理反应并且非转基因植物没有产量损失。因此，过表达GmMYB3a 的植物表现出更好的光合性能、更高的相对含水量和更高的水分利用效率，表明它们对缺水的适应能力。我们得出结论，GmMYB3a 的过表达可以提高花生的耐旱性和生产力。