Red rot caused by Colletotrichum falcatum poses a serious threat to sugarcane cultivation in many tropical and sub-tropical countries. Deciphering the molecular network of major defense-signaling pathways in sugarcane cultivars with varying red rot resistance is essential to elucidate the phenomenon of defense priming exerted by resistance inducers. Therefore, in this study, expression pattern of transcripts coding for major defense-signaling pathway regulatory genes was profiled during compatible and incompatible interactions and in response to defense priming using qRT-PCR. Candidate genes that were profiled are involved in or related to hypersensitive response and reactive oxygen species production (HR/ROS), salicylic acid (SA), and jasmonic acid/ethylene (JA/ET) pathways. For compatible and incompatible interactions, susceptible (CoC 671), field tolerant (Co 86032) and resistant (Co 93009) sugarcane cultivars were used, whereas for defense priming, benzothiadiazole (BTH) and the pathogen-associated molecular patterns (PAMPs) of C. falcatum viz., CfEPL1 (eliciting plant response-like) and CfPDIP1 (plant defense inducing protein) were used in CoC 671 cultivar. Results indicated that the master regulator of defense pathways, nonexpressor of pathogenesis-related genes 1 (NPR1) was highly upregulated in incompatible interactions (in both Co 86032 and Co 93009) than the compatible interaction along with SA pathway-associated genes. Similarly, in response to defense priming with BTH, CfEPL1 and CfPDIP1, only the SA pathway-associated genes showed considerable upregulation at 0 h post inoculation (hpi) and other intermittent time points. Overall, this study showed that SA-mediated defense pathway is the most predominant pathway reprogrammed during priming with BTH, CfEPL1 and CfPDIP1 and substantiated the earlier findings that these agents indeed induce systemic resistance against red rot of sugarcane.

炭疽菌引起的红腐病在许多热带和亚热带国家，甘蔗种植受到严重威胁。破解具有不同红腐病抗性的甘蔗品种中主要防御信号通路的分子网络，对于阐明抗性诱导物产生的防御启动现象至关重要。因此，在这项研究中，在兼容和不兼容的相互作用过程中以及响应于使用qRT-PCR的防御启动反应中，分析了编码主要防御信号通路调节基因的转录本的表达模式。分析的候选基因与超敏反应和活性氧产生（HR / ROS），水杨酸（SA）和茉莉酸/乙烯（JA / ET）途径有关或相关。对于兼容和不兼容的交互，敏感（CoC 671），C. falcatum即。，CfEPL1（引起植物反应样）和CfPDIP1（植物防御诱导蛋白）被用于CoC 671品种。结果表明，与SA途径相关基因相比，在不相容相互作用（Co 86032和Co 93009）中，防御途径的主要调节因子，即与发病机理相关的基因1（NPR1）的非表达显着上调。同样，响应BTH，CfEPL1和CfPDIP1的防御启动，只有SA途径相关基因在接种后0小时（hpi）和其他间歇时间点显示出明显的上调。总体而言，这项研究表明，SA介导的防御途径是BTH引发过程中最主要的重编程途径，