Background

Excessive sunlight exposure leads to photodamaged skin, resulting in wrinkles, roughness, relaxation, and pigmentation. We examined photoprotective effects of aqueous extracts of dried Phragmites communis rhizome (PCWE) on ultraviolet B radiation (UVB)-mediated photodamage in skin cells.

Methods

Human dermal fibroblasts (HDFs), melanocytes (B16F10 cells), and keratinocytes (HaCaT cells) were treated with PCWE (25–200 μg/mL), with or without UVB (30 mJ/cm²). Cell viability, cell senescence, and mRNA levels of genes involved in skin homeostasis were assessed. Anti-melanogenic effects of PCWE on B16F10 cells were evaluated. Cyclooxygenase-2 (COX-2) mRNA levels and β-hexosaminidase release were evaluated in macrophage RAW264.7 and basophilic leukemia RBL-2H3 cells, respectively.

Results

No significant cytotoxicity was observed in tested cells up to 200 μg/mL PCWE. In HDFs and HaCaT cells, PCWE pretreatment afforded significant, concentration-dependent photoprotection. PCWE downregulated baseline matrix metalloprotease-1 expression and elastase activity in HDFs; in HaCaT cells, telomerase reverse transcriptase and hyaluronan synthase-2 expressions were upregulated. PCWE suppressed α-melanocyte-stimulating hormone-mediated increase in melanin production and tyrosinase activity. PCWE suppressed COX-2 induction (in RAW264.7) and β-hexosaminidase release (in RBL-2H3).

Conclusion

PCWE exhibits good potential to attenuate photodamage in skin cells.

中文翻译：

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芦苇的水提取物是根茎减弱皮肤细胞的光毒性

背景

过度暴露在阳光下会导致皮肤光损伤，从而导致皱纹，粗糙，松弛和色素沉着。我们检查了干燥的芦苇根茎（PCWE）的水提物对皮肤细胞中紫外线B辐射（UVB）介导的光损伤的保护作用。

方法

用PCWE（25–200μg/ mL）或无UVB（30 mJ / cm ²）处理人皮肤成纤维细胞（HDF），黑素细胞（B16F10细胞）和角质形成细胞（HaCaT细胞）。评估了与皮肤稳态有关的细胞活力，细胞衰老和基因的mRNA水平。评估了PCWE对B16F10细胞的抗黑色素生成作用。分别在巨噬细胞RAW264.7和嗜碱性白血病RBL-2H3细胞中评估了环氧合酶2（COX-2）mRNA的水平和β-己糖胺酶的释放。

结果

在高达200μg/ mL PCWE的测试细胞中未观察到明显的细胞毒性。在HDF和HaCaT细胞中，PCWE预处理可提供明显的浓度依赖性光保护。PCWE下调了HDF中的基线基质金属蛋白酶-1表达和弹性蛋白酶活性；在HaCaT细胞中，端粒酶逆转录酶和透明质酸合酶2的表达上调。PCWE抑制了α-黑素细胞刺激激素介导的黑色素生成和酪氨酸酶活性的增加。PCWE抑制了COX-2诱导（在RAW264.7中）和β-己糖胺酶释放（在RBL-2H3中）。

结论

PCWE具有减弱皮肤细胞光损伤的良好潜力。