Several experimental models including patient biopsies, animal models, and cell lines have been recommended to study the mechanism of bladder cancer development. After several passages in culture, cell lines lose some original features, and no longer resemble the cells of their original tumor. This makes it necessary to establish various cell lines. In an attempt to establish a new cell line for bladder cancer, JAM-ICR (RRID: CVCL_A9QB) was derived from a 64-year-old man diagnosed with a high-grade tumor. This cell line was characterized in multiple experiments involving morphological studies, immunophenotyping (by immunohistochemistry and flow cytometry), karyotyping, short tandem repeat analysis, colony-forming assays, migration and invasion assays, and chemosensitivity to anti-cancer drugs. JAM-ICR cells are pale with an irregular polygonal shape, and show some similarities to mesenchymal stem cells but with a wider shape and shorter arms. Phenotypic assessment demonstrated the simultaneous expression of mesenchymal—(vimentin, desmin, CD29, CD90, and CD106) and epithelial lineage (pan-cytokeratin) markers, which supports a phenotype similar to epithelial–mesenchymal transition for this cell line. JAM-ICR displayed high metastatic potential and stem-like properties, i.e., self-renewal, colony forming, and the coexpression of TRA-1 with CD44 and CD166. Furthermore, this cell line was significantly more resistant to doxorubicin in comparison to the 5637 cell line. These features make JAM-ICR a new bladder cancer cell line with metastatic potential and stem-like properties, which may be potentially useful as a model to elucidate the molecular and cellular mechanisms of bladder cancer pathogenesis or evaluate new drugs.

包括患者活检、动物模型和细胞系在内的几种实验模型已被推荐用于研究膀胱癌发展的机制。在培养几次后，细胞系失去了一些原始特征，不再像它们原始肿瘤的细胞。这使得有必要建立各种细胞系。为了建立一种新的膀胱癌细胞系，JAM-ICR（RRID：CVCL_A9QB）源自一名被诊断患有高级别肿瘤的 64 岁男性。该细胞系在多个实验中得到表征，包括形态学研究、免疫表型分析（通过免疫组织化学和流式细胞术）、核型分析、短串联重复分析、集落形成分析、迁移和侵袭分析以及对抗癌药物的化学敏感性。JAM-ICR 细胞苍白，呈不规则多边形，与间充质干细胞有一些相似之处，但形状更宽，臂更短。表型评估表明间充质（波形蛋白、结蛋白、CD29、CD90 和 CD106）和上皮谱系（泛细胞角蛋白）标志物的同时表达，这支持与该细胞系的上皮-间充质转化相似的表型。JAM-ICR 显示出高转移潜能和茎样特性，即自我更新、集落形成以及 TRA-1 与 CD44 和 CD166 的共表达。此外，与 5637 细胞系相比，该细胞系对多柔比星的抗性明显更强。这些特征使 JAM-ICR 成为具有转移潜能和干细胞样特性的新型膀胱癌细胞系，