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The frequency of high-risk human papillomavirus types, HPV16 lineages, and their relationship with p16INK4a and NF-κB expression in head and neck squamous cell carcinomas in Southwestern Iran
Brazilian Journal of Microbiology ( IF 2.2 ) Pub Date : 2020-11-09 , DOI: 10.1007/s42770-020-00391-1
Fatemeh Pakdel 1, 2 , Ali Farhadi 1 , Tahereh Pakdel 1 , Azadeh Andishe-Tadbir 3 , Parnian Alavi 4 , Abbas Behzad-Behbahani 1 , Mohammad J Ashraf 5
Affiliation  

High-risk human papillomaviruses (hr-HPVs) are the key risk factors implicated in the development of a significant proportion of head and neck squamous cell carcinomas (HNSCCs). We aimed to investigate the distribution of hr-HPV types and HPV16 lineages in a sample of patients with HNSCC and the possible association between HPV status and the expression of P16INK4A and NF-κB in Iranian HNSCC patients. We examined 108 formalin-fixed, paraffin-embedded (FFPE) histologically confirmed primary SCC tissue specimens of different head and neck anatomical sites. HPV types and HPV16 lineages were determined by nested PCR and overlapping nested PCR assays, respectively, followed by gene sequencing and phylogenetic analysis. The expression of p16INK4a and NF-κB was evaluated by immunohistochemistry. Twenty-five (23.1%) HNSCC tissue specimens were tested positive for HPV infection. The most prevalent HPV type was HPV-16, followed by HPV18 and HPV11. HPV16 variants belonged to the lineage A and lineage D which were further sorted into sublineages A1, A2, and D2. A significant association between HPV status and p16INK4a immunoreactivity was observed in more than 76% of the HPV-related HNSCCs (P < 0.0001). The overexpression of p16INK4a and cytoplasmic NF-κB was more common in low-grade HNSCC tumors. Our data highlights that HPV16, in particular the A2 sublineage, followed by A1 and D2 sublineages are the major agents associated with HNSCCs in Iran. Based on HPV16 predominance and its lineage distribution pattern, it seems that the prophylactic vaccines developed for cervical cancer prevention could also be applicable for the prevention of HPV-related HNSCCs in our population.

中文翻译:

伊朗西南部头颈部鳞状细胞癌中高危人乳头瘤病毒类型、HPV16 谱系的频率及其与 p16INK4a 和 NF-κB 表达的关系

高危人乳头瘤病毒 (hr-HPV) 是与很大比例的头颈部鳞状细胞癌 (HNSCC) 发展有关的关键风险因素。我们旨在调查 HNSCC 患者样本中 hr-HPV 类型和 HPV16 谱系的分布,以及伊朗 HNSCC 患者中 HPV 状态与 P16INK4A 和 NF-κB 表达之间的可能关联。我们检查了 108 份福尔马林固定石蜡包埋 (FFPE) 组织学证实的不同头颈部解剖部位的原发性 SCC 组织标本。HPV 类型和 HPV16 谱系分别通过巢式 PCR 和重叠巢式 PCR 测定确定,然后进行基因测序和系统发育分析。通过免疫组织化学评估 p16INK4a 和 NF-κB 的表达。二十五 (23. 1%) HNSCC 组织标本检测为 HPV 感染阳性。最流行的 HPV 类型是 HPV-16,其次是 HPV18 和 HPV11。HPV16 变体属于谱系 A 和谱系 D,它们进一步分为亚谱系 A1、A2 和 D2。在超过 76% 的 HPV 相关 HNSCC 中观察到 HPV 状态与 p16INK4a 免疫反应性之间的显着关联(P < 0.0001)。p16INK4a 和细胞质 NF-κB 的过度表达在低级别 HNSCC 肿瘤中更为常见。我们的数据突出显示,HPV16,特别是 A2 亚系,其次是 A1 和 D2 亚系,是与伊朗 HNSCC 相关的主要病原体。基于 HPV16 优势及其谱系分布模式,
更新日期:2020-11-09
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