Broad-spectrum antibodies can effectively recognize substances with similar structures and have broad application prospects in field rapid detection. In this study, broad-spectrum antibodies (Abs) against organophosphorus pesticides (OPs) were used as sensitive recognition elements, which could effectively recognize most OPs. Gold nanoparticles (AuNPs) have good biocompatibility. It combined with Abs to form a gold-labeled probe (AuNPs–Abs), which enhances the effective binding of antibodies to nanomaterials. Prussian blue (PB) was added to electrodeposition solution to enhance the conductivity, resulting in superior electrochemical performance. The AuNP–Abs–PB composite film was prepared by electrodeposition on the electrode surface to improve the anti-interference ability and stability of the immunosensor. Under the optimal experimental conditions, the immunosensor had a wide detection range (IC₂₀–IC₈₀: 1.82 × 10^–3–3.29 × 10⁴ ng/mL) and high sensitivity. Most importantly, it was simple to be prepared and could be used to detect multiple OPs.

广谱抗体能有效识别结构相似的物质，在现场快速检测中具有广阔的应用前景。本研究以有机磷农药（OPs）的广谱抗体（Abs）为敏感识别元件，可有效识别大部分有机磷农药。金纳米粒子 (AuNPs) 具有良好的生物相容性。它与抗体结合形成金标记探针（AuNPs-Abs），增强了抗体与纳米材料的有效结合。将普鲁士蓝 (PB) 添加到电沉积溶液中以提高电导率，从而获得优异的电化学性能。通过在电极表面电沉积制备AuNP-Abs-PB复合膜，以提高免疫传感器的抗干扰能力和稳定性。₂₀ –IC ₈₀ : 1.82 × 10 ^–3 –3.29 × 10 ⁴  ng/mL) 和高灵敏度。最重要的是，它易于准备并可用于检测多个 OP。