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Tuning silica nanophotonics based on fluorescence resonance energy transfer for targeted non-classical light delivery applications
Journal of Nanophotonics ( IF 1.5 ) Pub Date : 2020-11-05 , DOI: 10.1117/1.jnp.14.046007
Carina Salinas 1 , Martin Amé 2 , A. Guillermo Bracamonte 1
Affiliation  

Abstract. Tunable fluorescent silica nanoparticles (SiO2 NPs) with variable sizes based on fluorescence resonance energy transfer were synthesized. The SiO2 NPs were obtained by the Störber method by varying tetraethyl orthosilicate (TEOS) concentrations and with the incorporation of different concentrations of fluorescein (Fl) and rhodamine B (RhB) conjugated with 3-(aminopropyl)triethoxysilane. We thus recorded homogeneous SiO2 NPs of different sizes. By transmission electron microscopy imaging, the sizes of 200, 280, and 380 nm were determined. The Fl and RhB fluorescent dyes showed well-overlapped emission from the fluorescent energy donor to the energy acceptor with the optimal ratio of quantum yields. By static fluorescence, varied emissions were recorded according to the concentration ratio of the donor–acceptor pair. Increasing intensity values were collected with the addition of higher concentrations of the fluorescent energy donor and decreased fluorescent lifetime decays. By laser fluorescence microscopy, higher enhanced surfaces were produced in the presence of both emitters, as compared with those in the presence of mono-colored SiO2 NPs with optimal excitation with the fluorescent energy donor only. These NPs were well dispersed in polar solvents; however, due to their polar surface and size, higher interactions produced dimeric nanoaggregates. In addition, to evaluate their applications, their depositions were evaluated over modified glass slide substrates for smart light-responsive materials and over Cyanobacteria and Escherichia coli for the development of nanobiostructures with varied optical activities.

中文翻译:

基于荧光共振能量转移的调谐二氧化硅纳米光子用于靶向非经典光传输应用

摘要。合成了基于荧光共振能量转移的具有可变尺寸的可调荧光二氧化硅纳米粒子 (SiO2 NPs)。通过改变原硅酸四乙酯 (TEOS) 浓度并加入不同浓度的荧光素 (Fl) 和罗丹明 B (RhB) 与 3-(氨基丙基)三乙氧基硅烷共轭,通过 Störber 方法获得 SiO2 NP。因此,我们记录了不同尺寸的均质 SiO2 NP。通过透射电子显微镜成像,确定了 200、280 和 380 nm 的尺寸。Fl 和 RhB 荧光染料显示出从荧光能量供体到能量受体的良好重叠发射,具有最佳的量子产率比。通过静态荧光,根据供体-受体对的浓度比记录不同的发射。通过添加更高浓度的荧光能量供体和减少的荧光寿命衰减来收集增加的强度值。通过激光荧光显微镜,与仅用荧光能量供体进行最佳激发的单色 SiO2 NPs 存在的情况相比,在两种发射体的存在下产生了更高的增强表面。这些纳米颗粒很好地分散在极性溶剂中;然而,由于它们的极性表面和尺寸,更高的相互作用会产生二聚体纳米聚集体。此外,为了评估它们的应用,在用于智能光响应材料的改性玻璃载玻片基板上以及在蓝藻和大肠杆菌上评估了它们的沉积物,以开发具有不同光学活性的纳米生物结构。
更新日期:2020-11-05
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