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The Expression of ZnT3 and GFAP Is Potentiated in the Hippocampus of Drug-Resistant Epileptic Rats Induced by Amygdala Kindling
Neuroimmunomodulation ( IF 2.4 ) Pub Date : 2020-11-06 , DOI: 10.1159/000510399
Yuanxin Huang 1 , Lin Wang 2 , Siying Ren 3 , Guofeng Wu 3 , Jing Wu 4
Affiliation  

Objective: The first-line treatment for epilepsy, a chronic neurological disorder characterized by spontaneous seizures, includes the application of anticonvulsant drug therapy. Only one-third of patients are incapable of complete controlling of their seizures after the administration of ≥2 pharmaceuticals. Here, we aimed to observe the ultrastructure changes and the expression of ZnT3 and GFAP in the hippocampus of drug-resistant epileptic rats. Methods: A total of 50 healthy adult male SD rats were used to generate the model ofepilepsy by amygdala kindling. After the rats were successfully kindled, pharmacoresistant epileptic (PRE) rats were selected according to their response to phenobarbital and phenytoin. The ultrastructure as well as the expression of zinc transporter 3 (ZnT3, a member of a growing family of mammalian zinc transporters) and glial fibrillary acidic protein (GFAP) were compared among PRE, pharmacosensitive epileptic (PRE), and normal (NRC) rats. Results: The PRE rats displayed severe synapses, neuronal degeneration, and necrosis. Moreover, the expression of ZnT3 and GFAP was significantly increased in both PRE and PSE rats; compared with NRC rats, the promotion of this expression was more pronounced in the PRE rats. Conclusions: Taken together, obvious synapses, neuronal degeneration, necrosis, mossy fiber sprouting, and astrogliosis were found in the drug-resistant epileptic rat model induced by amygdala kindling.
Neuroimmunomodulation


中文翻译:

杏仁核点燃诱导耐药癫痫大鼠海马区ZnT3和GFAP表达增强

目的:癫痫是一种以自发性癫痫发作为特征的慢性神经系统疾病,其一线治疗包括应用抗惊厥药物治疗。服用≥2种药物后,只有三分之一的患者无法完全控制癫痫发作。本研究旨在观察耐药癫痫大鼠海马组织中ZnT3和GFAP的超微结构变化及表达情况。方法:采用健康成年雄性SD大鼠共50只制作杏仁核点燃癫痫。成功点燃大鼠后,根据其对苯巴比妥和苯妥英的反应选择药物抗性癫痫(PRE)大鼠。比较了 PRE、药物敏感癫痫 (PRE) 和正常 (NRC) 大鼠的超微结构以及锌转运蛋白 3(ZnT3,一个不断增长的哺乳动物锌转运蛋白家族的成员)和神经胶质纤维酸性蛋白 (GFAP) 的表达. 结果: PRE 大鼠表现出严重的突触、神经元变性和坏死。此外,ZnT3和GFAP在PRE和PSE大鼠中的表达均显着增加;与 NRC 大鼠相比,这种表达的促进在 PRE 大鼠中更为明显。结论:综上所述,杏仁核点燃诱导的耐药癫痫大鼠模型出现明显的突触、神经元变性、坏死、苔藓纤维发芽和星形胶质细胞增生。
神经免疫调节
更新日期:2020-11-06
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