Abstract

The complete mitochondrial genome of the doubled-lined mackerel, Grammatorcynus bilineatus, was determined by the combination of high-throughput sequencing (HTS) and Sanger sequencing. The constructed mitochondrial genome of G. bilineatus was 16,537 bp in length, which harbors a canonical 37 genes (13 proteins, two ribosomal RNAs, and 22 tRNAs) and two non-coding regions (origin of light-strand replication (O_L) and the D-loop control region). Among 38 genes, nine were encoded on its light strand (L), while the other 28 were on its heavy strand (H). Besides COX1 (GTG) and ATP6 (CTG), the other eleven protein-coding genes (PCGs) begin with a typical start codon (ATG). The phylogenetic tree showed that G. bilineatus was not clustered with the other species in the Scombridae, forming a clade for Grammatorcynus. The genetic information of G.bilineatus will provide useful information for the scientific management and conservation of the species in the genus.

摘要

通过高通量测序（HTS）和Sanger测序相结合，确定了双线鲭鱼Grammatorcynus bilineatus的完整线粒体基因组。的构造的线粒体基因组G. bilineatus的长度为16537个碱基，其藏着一个规范37个基因（13种蛋白质中，两个核糖体RNA，和22个tRNA）和两个非编码区的光链复制（O（原点_大号）和D回路控制区域）。在38个基因中，有9个在其轻链（L）上编码，而其他28个在其重链（H）上编码。除COX1（GTG）和ATP6（CTG）外，其他十一个蛋白质编码基因（PCG）以典型的起始密码子（ATG）开头。系统发育树表明双歧线虫在不与鲭科的其他物种聚集，形成一个分支Grammatorcynus。G.bilineatus的遗传信息将为科学管理和保护该属中的物种提供有用的信息。