The aggregate formation and stability are controlled by the dynamics of soil organic matters (SOM), but how it is related to SOM chemical composition within different‐sized aggregates is largely unknown during manure fertilization. In this study, the variations of intra‐aggregate organic carbon (OC), including intra‐particulate organic matter (iPOM) and mineral‐associated organic matter, were quantitatively and qualitatively analysed, and then, their effects on aggregate formation and stability were assessed under four treatments: control (CK), mineral fertilizer (NPK), reduced manure (30%M) and manure fertilizers (M). Manure application (M) significantly increased macroaggregate proportion, mean weight diameter (MWD), and OC contents within different‐sized aggregates compared to CK, NPK, and 30%M. The OC accumulation of macroaggregate in M was attributed to OC content increase in silt plus clay subfraction rather than iPOM with more labile organic groups; oppositely, in microaggregate it was located in the relatively stable fine iPOM. The macroaggregate formation and stability were controlled by the fine iPOM within macroaggregates, whose abundant polysaccharide‐C and aliphatic‐C after manure fertilization advanced the microbial growth except for Gram‐positive bacteria, which further promoted macroaggregate formation and stability. The free silt plus clay fraction also affected macroaggregate formation and stability, and its polysaccharide‐C derived from microorganisms or decomposing SOM was positively associated with MWD and macroaggregate proportion. Because polysaccharide‐C can be easily associated with mineral particles, further improving micro‐ or macroaggregation. We conclude that continuous manure fertilization could increase labile SOM accumulation within aggregates and then facilitate microbial growth, which collectively are responsible for aggregate formation and stabilization.

中文翻译：

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潮土有机肥施肥过程中团聚体内部有机碳的变化影响团聚体的形成和稳定性

团聚体的形成和稳定性受土壤有机质（SOM）的动力学控制，但是在肥料施肥过程中，其与不同尺寸团聚体中SOM化学成分之间的关​​系尚不清楚。在这项研究中，定量和定性分析了集内有机碳（OC）的变化，包括颗粒内有机物（iPOM）和矿物相关有机物，然后评估了它们对集聚体形成和稳定性的影响。四种处理方式：对照（CK），矿物肥料（NPK），减少的肥料（30％M）和肥料（M）。与CK，NPK和30％M相比，施肥（M）显着增加了不同尺寸骨料中大骨料的比例，平均重径（MWD）和OC含量。M中大骨料的OC积累归因于淤泥加黏土分馏物中的OC含量增加，而不是有机基团较不稳定的iPOM。相反，在微骨料中，它位于相对稳定的精细iPOM中。大型骨料的形成和稳定性受精细骨料中iPOM的控制，粪肥施肥后其丰富的多糖-C和脂肪族-C促进了除革兰氏阳性菌外的微生物生长，从而进一步促进了大型骨料的形成和稳定性。游离粉砂和粘土组分也影响大骨料的形成和稳定性，其来源于微生物或分解SOM的多糖C与MWD和大骨料的比例呈正相关。由于多糖C很容易与矿物质颗粒结合，进一步改善微观或宏观聚合。我们得出的结论是，连续施肥可以增加骨料中不稳定的SOM积累，然后促进微生物的生长，而这些微生物共同负责骨料的形成和稳定。