The cryosurvival of embryos is a complex process involving dynamic and integrated morphological, functional, and molecular changes. Here, we evaluated the transcriptional profiling of bovine embryos possessing high and low cryotolerance (HC and LC, respectively) by assessing the resumption of development. Embryos were produced in vitro (N = 1137) and cryopreserved (N = 894). Blastocysts samples possessed pronounced group individualization at RNA sequencing. A total of 114 genes were differentially expressed, and 27 and 84 genes were upregulated in HC and LC, respectively. Among the over‐represented biological functions, cellular growth and proliferation, cell death and survival, and organismal survival were predicted to be activated, while cellular movement and cell‐to‐cell signaling were predicted to be inhibited in HC embryos. Enriched canonical pathways and upstream regulators related to cellular proliferation and survival (HC), inflammatory processes, and cell death (LC) were predicted to represent two embryonic molecular profiles present during the resumption of development after cryopreservation. The marked contrast in transcriptional profiles between HC and LC strongly suggests the influence of embryonic competence after cryopreservation on its respective transcriptome and indicated that HC and LC presented two different molecular strategies to overcome cryopreservation‐related stress and resume postcryopreservation development.

中文翻译：

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胚胎低温耐受性的转录分析

胚胎的冷冻存活是一个复杂的过程，涉及动态和综合的形态、功能和分子变化。在这里，我们通过评估发育的恢复来评估具有高和低低温耐受性（分别为 HC 和 LC）的牛胚胎的转录谱。胚胎在体外产生 ( N  = 1137) 并冷冻保存 ( N = 894）。囊胚样本在 RNA 测序中具有明显的群体个体化。共有 114 个基因差异表达，分别在 HC 和 LC 中上调了 27 个和 84 个基因。在过度表达的生物学功能中，细胞生长和增殖、细胞死亡和存活以及生物体存活被预测为被激活，而细胞运动和细胞间信号被预测在 HC 胚胎中被抑制。预计与细胞增殖和存活 (HC)、炎症过程和细胞死亡 (LC) 相关的丰富经典途径和上游调节因子代表了冷冻保存后恢复发育过程中存在的两种胚胎分子谱。