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Effect of silver nanoparticles (AgNPs) exposure on microRNA expression and global DNA methylation in endothelial cells EA.hy926
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2020-11-06 , DOI: 10.1016/j.etap.2020.103543
A.K. González-Palomo , K. Saldaña-Villanueva , J.D. Cortés-García , J.C. Fernández-Macias , K.B. Méndez-Rodríguez , I.N. Pérez Maldonado

The aim of this study was to determine the effect of AgNPs on the epigenome of endothelial cells EA.hy926, including the levels of expression of microRNAs (miRNAs) and global DNA methylation patterns. In addition, evaluation of the expression of inflammatory genes and the levels of VCAM-1 protein (miRNA-126 target) was performed. The expression levels of analyzed miRNAs (microRNAs-126, 155 and 146) were reduced significantly and there were not observed changes in inflammatory gene expression. Regarding the levels of protein vascular cell adhesion molecule 1 (VCAM-1), they increase significantly to 0.5 μM AgNPs at 24 h of exposure. As far as DNA methylation is concerned, we found that AgNPs induce a state of global hyper-methylation. In conclusion, it was demonstrated that direct contact between AgNPs and endothelial cells resulted in the dysregulation of highly enriched and vastly functional miRNAs and DNA hypermethylation, that may have multiple effects on endothelium function and integrity.



中文翻译:

纳米银(AgNPs)暴露对内皮细胞EA.hy926中microRNA表达和整体DNA甲基化的影响

这项研究的目的是确定AgNPs对内皮细胞EA.hy926表观基因组的影响,包括microRNA(miRNA)的表达水平和整体DNA甲基化模式。此外,还评估了炎症基因的表达和VCAM-1蛋白(miRNA-126靶标)的水平。分析的miRNA(microRNA-126、155和146)的表达水平显着降低,并且未观察到炎症基因表达的变化。关于蛋白质血管细胞粘附分子1(VCAM-1)的水平,在暴露24 h时,它们显着增加至0.5μMAgNP。就DNA甲基化而言,我们发现AgNPs诱导了全局超甲基化状态。结论,

更新日期:2020-11-12
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