Lysolipid-containing thermosensitive liposomes (LTSL) have gained attention for triggered release of chemotherapeutics. Superparamagnetic iron oxide nanoparticles (SPION) offers multimodal imaging and hyperthermia therapy opportunities as a promising theranostic agent. Combining LTSL with SPION may further enhance their performance and functionality of LTSL. However, a major challenge in clinical translation of nanomedicine is the poor scalability and complexity of their preparation process. Exploiting the nature of self-assembly, nanoprecipitation is a simple and scalable technique for preparing liposomes. Herein, we developed a novel SPION-incorporated lysolipid-containing thermosensitive liposome (mLTSL10) formulation using nanoprecipitation. The formulation and processing parameters were carefully designed to ensure high reproducibility and stability of mLTSL10. The effect of solvent, aqueous-to-organic volume ratio, SPION concentration on the mLTSL10 size and dispersity was investigated. mLTSL10 were successfully prepared with a small size (∼100 nm), phase transition temperature at around 42 °C, and high doxorubicin encapsulation efficiency. Indifferent from blank LTSL, we demonstrated that mLTSL10 combining the functionality of both LTSL and SPION can be successfully prepared using a scalable nanoprecipitation approach.

含溶脂的热敏脂质体（LTSL）已引起化学治疗药物的触发释放。超顺磁性氧化铁纳米粒子（SPION）提供了多模式成像和热疗治疗机会，是一种很有前途的治疗剂。将LTSL与SPION结合使用可以进一步增强LTSL的性能和功能。然而，纳米医学临床翻译中的主要挑战是其制备过程的差的可扩展性和复杂性。利用自组装的本质，纳米沉淀是制备脂质体的一种简单且可扩展的技术。本文中，我们使用纳米沉淀技术开发了一种新型的掺入SPION的含溶血脂的热敏脂质体（mLTSL10）制剂。精心设计了配方和加工参数，以确保mLTSL10的高重现性和稳定性。研究了溶剂，水与有机物的体积比，SPION浓度对mLTSL10大小和分散度的影响。mLTSL10已成功制备，具有小尺寸（〜100 nm），相变温度约42°C和高阿霉素包封效率。与空白LTSL无关，我们证明了可以使用可扩展的纳米沉淀方法成功制备结合了LTSL和SPION功能的mLTSL10。且阿霉素的包封效率高。与空白LTSL无关，我们证明了可以使用可扩展的纳米沉淀方法成功制备结合了LTSL和SPION功能的mLTSL10。且阿霉素的包封效率高。与空白LTSL无关，我们证明了可以使用可扩展的纳米沉淀方法成功制备结合了LTSL和SPION功能的mLTSL10。