In the current study, the synthesis of a theranostic platform composed of superparamagnetic iron oxide nanoparticles (SPION)-deferasirox conjugates targeted with AS1411 DNA aptamer was reported. In this regard, SPION was amine-functionalized by (3-aminopropyl)trimethoxysilane (ATPMS), and then deferasirox was covalently conjugated onto its surface. Finally, to provide guided drug delivery to cancerous tissue, AS1411 aptamer was conjugated to the complex of SPION-deferasirox. The cellular toxicity assay on CHO, C-26 and AGS cell lines verified higher cellular toxicity of targeted complex in comparison with non-targeted one. The evaluation of in vivo tumor growth inhibitory effect in C26 tumor-bearing mice illustrated that the aptamer-targeted complex significantly enhanced the therapeutic outcome in comparison with both non-targeted complex and free drug. The diagnostic capability of the prepared platform was also evaluated implementing C26-tumor-bearing mice. Obtained data confirmed higher tumor accumulation and higher tumor residence time for targeted complex through MRI imaging due to the existence of SPION as a contrast agent in the core of the prepared complex. The prepared multimodal theranostic system provides a safe and effective platform for fighting against cancer.

在目前的研究中，报道了由超顺磁性氧化铁纳米粒子 (SPION)-地拉罗司结合物组成的治疗诊断平台的合成，该结合物以 AS1411 DNA 适配体为目标。在这方面，SPION 被（3-氨基丙基）三甲氧基硅烷 (ATPMS) 胺官能化，然后将地拉罗司共价结合到其表面。最后，为了向癌组织提供引导药物递送，AS1411 适体与 SPION-地拉罗司复合物结合。CHO、C-26 和 AGS 细胞系的细胞毒性试验证实，与非靶向复合物相比，靶向复合物具有更高的细胞毒性。对 C26 荷瘤小鼠体内肿瘤生长抑制作用的评估表明，与非靶向复合物和游离药物相比，适体靶向复合物显着增强了治疗效果。还评估了实施 C26 荷瘤小鼠的准备平台的诊断能力。由于在制备的复合物的核心中存在作为造影剂的 SPION，获得的数据通过 MRI 成像证实了靶向复合物的更高的肿瘤积累和更长的肿瘤停留时间。所制备的多模式治疗诊断系统为抗击癌症提供了一个安全有效的平台。由于在制备的复合物的核心中存在作为造影剂的 SPION，获得的数据通过 MRI 成像证实了靶向复合物的更高的肿瘤积累和更长的肿瘤停留时间。所制备的多模式治疗诊断系统为抗击癌症提供了一个安全有效的平台。由于在制备的复合物的核心中存在作为造影剂的 SPION，获得的数据通过 MRI 成像证实了靶向复合物的更高的肿瘤积累和更长的肿瘤停留时间。所制备的多模式治疗诊断系统为抗击癌症提供了一个安全有效的平台。