Glutathione (GSH) is an important bio-thiol which is playing vital role in the human system, such as treating glaucoma, preventing asthma, cancer, etc. Therefore, electrochemical sensing of GSH is becoming very vital to know the level of the biomolecule in living systems. Hence, the present work aims to develop a sensor matrix for electrochemical biosensing of GSH at Au nanoparticle decorated Nanoporous poly(3,4)ethylene dioxythiophene (PEDOT) modified glassy carbon electrode (AuNPPEDOT/ GCE). The electrode is modified with a nanoporous fibrillar network of PEDOT containing Au nanoparticles and characterized using UV-Visible spectroscopy and Field Emission Scanning Electron Microscopy (FESEM). The platform is capable of producing enhanced signal against the bio-analyte at the applied overpotential of 0.6 V. Whereas bare glassy carbon electrode is unable to produce the signal. Conversely, Nanoporous PEDOT modified glassy carbon electrode can sense GSH of very low sensitivity at an applied overpotential of 0.7 V. From single step chronoamperometric measurements, kinetic parameters such as diffusion coefficient and apparent rate constant of the reaction were found as 5.01 × 10^-5 cm²·s^-1 and 1.106 × 10³ M^-1·s^-1 respectively. From the amperometric response, the sensitivity and limit of detection (LOD) of the electrode were to be 10.7μA cm^-2/μM and 0.173 μM respectively with the linear range of 0.5 to 10 μM. The matrix can detect GSH in the presence of other possible interfering molecules present in blood samples.

谷胱甘肽（GSH）是一种重要的生物硫醇，在人类系统中起着至关重要的作用，例如治疗青光眼，预防哮喘，癌症等。因此，对GSH的电化学检测对于了解生命系统中生物分子的水平变得至关重要。因此，本工作旨在开发一种传感器矩阵，用于在金纳米粒子修饰的纳米多孔聚（3,4）乙烯二氧噻吩（PEDOT）修饰的玻碳电极（AuNPPEDOT / GCE）上对GSH进行电化学生物传感。电极用含有Au纳米粒子的PEDOT纳米多孔原纤维网络修饰，并使用紫外可见光谱和场发射扫描电子显微镜（FESEM）进行表征。该平台能够在施加的0.6 V过电势下针对生物分析物产生增强的信号。而裸露的玻璃碳电极则无法产生该信号。反过来，^-5 cm ² ·s ^-1和1.106×10 ³ M ^-1 ·s ^-1。从安培响应，电极的灵敏度和检测限（LOD）分别为10.7μAcm ^-2 /μM和0.173μM ，线性范围为0.5至10μM。在血样中存在其他可能的干扰分子的情况下，基质可以检测GSH。