This study describes the optimization of a culture medium to improve protease production by Janthinobacterium sp. using response surface methodology (RSM) and comparative proteomic analysis. Using a one-variable-at-a-time approach, dextrose, Triton X-100, peptone, and pH were identified as significant factors affecting protease production. The optimized medium resulted in a 6.25-fold increase in protease activity when compared to that in the original medium. To investigate the metabolic changes in the optimized medium, extracellular and intracellular proteins were analyzed by two-dimensional gel electrophoresis. Some proteins essential to cell viability were highly expressed in the optimized medium and the outer membrane protein A was secreted at a higher level. These results can be useful for the industrial application of psychrophiles that produce cold-adapted enzymes.

这项研究描述了培养基的优化以提高Janthinobacterium产生蛋白酶的能力sp。使用响应面方法（RSM）和比较蛋白质组学分析。使用一次可变的方法，葡萄糖，Triton X-100，蛋白ept和pH被确定为影响蛋白酶生产的重要因素。与原始培养基相比，优化的培养基导致蛋白酶活性提高了6.25倍。为了研究优化培养基中的代谢变化，通过二维凝胶电泳分析了细胞外和细胞内蛋白。在优化培养基中高表达了一些对细胞活力至关重要的蛋白质，并且较高水平分泌了外膜蛋白A。这些结果可用于产生冷适应酶的嗜冷菌的工业应用。