Carotenoids, which have biologically beneficial effects and occur naturally in microorganisms and plants, are pigments widely applied in the food, cosmetics and pharmaceutical industries. The compound 4,4'-diaponeurosporene is a C₃₀ carotenoid produced by some Lactobacillus species, and L. plantarum is the main species producing it. In this study, the antioxidant activity of 4,4'-diaponeurosporene extracted from L. plantarum subsp. plantarum KCCP11226 was examined. Maximum carotenoid content (0.74 ± A₄₇₀) was obtained at a relatively low temperature (20°C). The DPPH radical scavenging ability of 4,4'-diaponeurosporene (1 mM) was approximately 1.7-fold higher than that of butylated hydroxytoluene (BHT), a well-known antioxidant food additive. In addition, the ABTS radical scavenging ability was shown to be 2.3- to 7.5-fold higher than that of BHT at the range of concentration from 0.25 mM to 1 mM. The FRAP analysis confirmed that 4,4'-diaponeurosporene (0.25 mM) was able to reduce Fe³⁺ by 8.0-fold higher than that of BHT. Meanwhile, 4,4'-diaponeurosporene has been confirmed to be highly resistant to various external stresses (acid/bile, high temperature, and lysozyme conditions). In conclusion, L. plantarum subsp. plantarum KCCP11226, which produces 4,4'-diaponeurosporene as a functional antioxidant, may be a potentially useful strain for the development of functional probiotic industries.

中文翻译：

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来自植物乳杆菌亚种的 4,4'-Diaponeurosporene。plantarum KCCP11226：低温应激诱导的生产增强和体外抗氧化活性。

类胡萝卜素具有生物学上的有益作用，天然存在于微生物和植物中，是广泛应用于食品、化妆品和制药行业的色素。化合物4,4'-diaponeurosporene是一种C ₃₀类胡萝卜素，由一些乳杆菌属产生，植物乳杆菌是主要产生它的物种。在这项研究中，从植物乳杆菌亚种中提取的 4,4'-diaponeurosporene 的抗氧化活性。检查了植物乳杆菌 KCCP11226。最大类胡萝卜素含量 (0.74 ± A ₄₇₀) 是在相对较低的温度 (20°C) 下获得的。4,4'-diaponeurosporene (1 mM) 的 DPPH 自由基清除能力比众所周知的抗氧化食品添加剂丁基化羟基甲苯 (BHT) 高约 1.7 倍。此外，在 0.25 mM 至 1 mM 的浓度范围内，ABTS 的自由基清除能力比 BHT 高 2.3 至 7.5 倍。FRAP 分析证实，4,4'-diaponeurosporene (0.25 mM) 能够将 Fe ³⁺还原为 BHT 的 8.0 倍。同时，已证实 4,4'-diaponeurosporene 对各种外部压力（酸/胆汁、高温和溶菌酶条件）具有高度抵抗力。总之，植物乳杆菌亚种。植物油KCCP11226 产生 4,4'-diaponeurosporene 作为功能性抗氧化剂，可能是开发功能性益生菌产业的潜在有用菌株。