A novel approach applying a conico-cylindrical flask (CCF) was used to study the growth of two morphologically identical Phormidium strains namely AP3 and AP9F. Further, the monosaccharide composition of the exopolysaccharide (EPS), its production, applicability in industrial processes and biological activity were evaluated. These evaluations were performed by employing contrasting cultivation modes (biofilm or planktonic) and different surface materials (hydrophobic polymethyl methacrylate (PMMA) and hydrophilic glass (G). Biofilm formation was seen to be elevated in PMMA-CCF cultivated Phormidium AP3 and AP9F than G-CCF mode. Among the two surface materials tested Phormidium AP3 and AP9F produced the highest amount of planktonic biomass in PMMA-CCF than in G-CCF mode. Average values of biofilm capsular/bound polysaccharide and polysaccharide released by cell suspension of Phormidium AP3 and AP9F were elevated in PMMA-CCF compared to the G-CCF setup. Protein concentrations in released polysaccharide (RPS) and biofilm of PMMA-CCF were higher in the Phormidium AP3 and AP9F when compared to the G-CCF cultivation. The antimicrobial, emulsifying and flocculating activities of EPS were assessed using standard assays.

中文翻译：

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表面材料对潮间带蓝藻Pormidium sp。的生长模式和生物活性外聚合物的影响。

一种应用圆锥形烧瓶（CCF）的新颖方法被用来研究两种形态相同的Ph菌株AP3和AP9F的生长。此外，评估了胞外多糖（EPS）的单糖组成，其生产，在工业过程中的适用性和生物活性。这些评估通过采用对比培养模式（生物膜或浮游）和不同的表面材料（疏水的聚甲基丙烯酸甲酯（PMMA）和亲水性玻璃（G）。生物膜形成被认为在PMMA-CCF中升高培养进行席藻AP3和AP9F除G -CCF模式。在这两个表面材料测试席藻与G-CCF模式相比，PMMA-CCF中AP3和AP9F产生的浮游生物量最高。生物膜荚膜/结合的多糖和多糖通过的细胞悬浮液释放的平均值席藻AP3和AP9F在PMMA-CCF均升高相比，G-CCF设置。在释放多糖（RPS）和PMMA-CCF的生物膜蛋白浓度在较高的席藻相比G-CCF栽培时AP3和AP9F。使用标准测定法评估EPS的抗菌，乳化和絮凝活性。