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Multi-Stimuli-Responsive DOX Released from Magnetosome for Tumor Synergistic Theranostics
International Journal of Nanomedicine ( IF 8 ) Pub Date : 2020-11-05 , DOI: 10.2147/ijn.s275655 Ming-Fong Tsai , Yu-Lun Lo , Yuan-Chun Huang , Chun-Chieh Yu , Yi-Ting Wu , Chia-Hao Su , Li-Fang Wang
International Journal of Nanomedicine ( IF 8 ) Pub Date : 2020-11-05 , DOI: 10.2147/ijn.s275655 Ming-Fong Tsai , Yu-Lun Lo , Yuan-Chun Huang , Chun-Chieh Yu , Yi-Ting Wu , Chia-Hao Su , Li-Fang Wang
Background: To improve responses to tumor microenvironments for achieving a better therapeutic outcome in combination cancer therapy, poly(ϵ-caprolactone)-SS-poly(methacrylic acid) diblock copolymer (PCL-SS-PMAA) with a disulfide linkage between the hydrophobic and hydrophilic junctions was synthesized.
Materials and Methods: Repeating units of PCL and PMAA in PCL-SS-PMAA were controlled and formulated into polymersomes (PSPps). Truncated octahedral Fe3O4 nanoparticles (IONPs) were synthesized and encapsulated to produce IONPs-PSPps NPs and doxorubicin (DOX) was further loaded to produce IONPs-PSPps@DOX NPs for theranostic applications.
Results: IONPs-PSPps NPs remained a superparamagnetic property with a saturation magnetization value of 85 emu⋅gFe3O4− 1 and a relaxivity value of 180 mM− 1⋅s− 1. Upon exposure to an alternating magnetic field (AMF), IONPs-PSPps NPs increased temperature from 25°C to 54°C within 15 min. Among test groups, the cell apoptosis was greatest in the group exposed to IONPs-PSPps@DOX NPs with AMF and magnet assistance. In vivo T2-weighted magnetic resonance images of A549 tumor-bearing mice also showed highest contrast and greatest tumor suppression in the tumor with AMF and magnet assistance.
Conclusion: IONPs-PSPps@DOX NPs are a potential theranostic agent having multifaceted applications involving magnetic targeting, MRI diagnosis, hyperthermia and chemotherapy.
中文翻译:
多刺激响应 DOX 从 Magnetosome 释放用于肿瘤协同治疗学
背景:为了提高对肿瘤微环境的反应以在联合癌症治疗中获得更好的治疗效果,聚(ε-己内酯)-SS-聚(甲基丙烯酸)二嵌段共聚物(PCL-SS-PMAA)在疏水性和疏水性之间具有二硫键。亲水结被合成。
材料和方法:控制 PCL-SS-PMAA 中 PCL 和 PMAA 的重复单元,并将其配制成聚合物囊体 (PSPps)。合成并封装截短的八面体 Fe 3 O 4纳米粒子 (IONPs) 以生产 IONPs-PSPps NPs,并进一步加载阿霉素 (DOX) 以生产用于治疗诊断应用的 IONPs-PSPps@DOX NPs。
结果:IONPs-PSPps NPs 仍然具有超顺磁性,饱和磁化强度值为 85 emu⋅g Fe3O4 - 1,弛豫值为 180 mM - 1 ⋅s - 1。在暴露于交变磁场 (AMF) 后,IONPs-PSPps NPs 在 15 分钟内将温度从 25°C 升高到 54°C。在测试组中,在 AMF 和磁体辅助下暴露于 IONPs-PSPps@DOX NPs 的组中细胞凋亡最大。A549 荷瘤小鼠的体内 T 2加权磁共振图像也显示在 AMF 和磁体辅助下的肿瘤中具有最高的对比度和最大的肿瘤抑制。
结论:IONPs-PSPps@DOX NPs 是一种潜在的治疗诊断剂,具有多方面的应用,包括磁靶向、MRI 诊断、热疗和化疗。
更新日期:2020-11-06
Materials and Methods: Repeating units of PCL and PMAA in PCL-SS-PMAA were controlled and formulated into polymersomes (PSPps). Truncated octahedral Fe3O4 nanoparticles (IONPs) were synthesized and encapsulated to produce IONPs-PSPps NPs and doxorubicin (DOX) was further loaded to produce IONPs-PSPps@DOX NPs for theranostic applications.
Results: IONPs-PSPps NPs remained a superparamagnetic property with a saturation magnetization value of 85 emu⋅gFe3O4− 1 and a relaxivity value of 180 mM− 1⋅s− 1. Upon exposure to an alternating magnetic field (AMF), IONPs-PSPps NPs increased temperature from 25°C to 54°C within 15 min. Among test groups, the cell apoptosis was greatest in the group exposed to IONPs-PSPps@DOX NPs with AMF and magnet assistance. In vivo T2-weighted magnetic resonance images of A549 tumor-bearing mice also showed highest contrast and greatest tumor suppression in the tumor with AMF and magnet assistance.
Conclusion: IONPs-PSPps@DOX NPs are a potential theranostic agent having multifaceted applications involving magnetic targeting, MRI diagnosis, hyperthermia and chemotherapy.
中文翻译:
多刺激响应 DOX 从 Magnetosome 释放用于肿瘤协同治疗学
背景:为了提高对肿瘤微环境的反应以在联合癌症治疗中获得更好的治疗效果,聚(ε-己内酯)-SS-聚(甲基丙烯酸)二嵌段共聚物(PCL-SS-PMAA)在疏水性和疏水性之间具有二硫键。亲水结被合成。
材料和方法:控制 PCL-SS-PMAA 中 PCL 和 PMAA 的重复单元,并将其配制成聚合物囊体 (PSPps)。合成并封装截短的八面体 Fe 3 O 4纳米粒子 (IONPs) 以生产 IONPs-PSPps NPs,并进一步加载阿霉素 (DOX) 以生产用于治疗诊断应用的 IONPs-PSPps@DOX NPs。
结果:IONPs-PSPps NPs 仍然具有超顺磁性,饱和磁化强度值为 85 emu⋅g Fe3O4 - 1,弛豫值为 180 mM - 1 ⋅s - 1。在暴露于交变磁场 (AMF) 后,IONPs-PSPps NPs 在 15 分钟内将温度从 25°C 升高到 54°C。在测试组中,在 AMF 和磁体辅助下暴露于 IONPs-PSPps@DOX NPs 的组中细胞凋亡最大。A549 荷瘤小鼠的体内 T 2加权磁共振图像也显示在 AMF 和磁体辅助下的肿瘤中具有最高的对比度和最大的肿瘤抑制。
结论:IONPs-PSPps@DOX NPs 是一种潜在的治疗诊断剂,具有多方面的应用,包括磁靶向、MRI 诊断、热疗和化疗。