Fluorescent nanodiamonds (fNDs) are emerging as important tools for imaging and sensing in biology, which enable the optical detection, for example, of temperature and magnetic fields at the nanoscale. At the same time, their unique physicochemical properties allow fNDs to drastically improve the properties of nanocomposites. Here, we report the integration of fNDs into electrospun poly-ε-caprolactone (PCL) fibers and the use of the resulting hybrid material as a nontoxic and multifunctional bioscaffold. We investigate the morphology, size distribution, optical properties, wettability, and biocompatibility of PCL fibers containing 0.2 and 0.4 wt % fNDs and demonstrate the quantum sensing capability of the nanocomposite via optically detected magnetic resonance measurements of the nitrogen-vacancy center in fNDs. We find that the use of ethanol as a cosolvent improves the dispersion of fNDs into PCL fibers and reduces the occurrence of fiber defects. We demonstrate that the PCL/fND scaffold is not cytotoxic for mesenchymal stem cells and even promotes cell adhesion and cell proliferation up to 21 days because of an increase in wettability compared to pure PCL fibers. Our results highlight the immense potential of PCL/fND nanocomposites as smart bioscaffolds for advanced biomedical applications such as tissue engineering, biosensing, and theranostics.

中文翻译：

---

嵌入生物医学支架的聚ε-己内酯纤维中的荧光纳米金刚石

荧光纳米金刚石（fNDs）作为生物学成像和传感的重要工具正在兴起，例如可以光学检测纳米级的温度和磁场。同时，它们独特的理化性质使fNDs可以大大改善纳米复合材料的性质。在这里，我们报告了将fNDs整合到电纺聚ε-己内酯（PCL）纤维中，并将所得杂化材料用作无毒且多功能的生物支架。我们研究了含有0.2和0.4 wt％fNDs的PCL纤维的形态，尺寸分布，光学性能，润湿性和生物相容性，并通过纳米线证明了纳米复合材料的量子感测能力。fNDs中氮空位中心的光学检测磁共振测量结果。我们发现使用乙醇作为助溶剂可改善fNDs在PCL纤维中的分散并减少纤维缺陷的发生。我们证明了PCL / fND支架对间充质干细胞没有细胞毒性，甚至比纯PCL纤维增加了润湿性，甚至可以促进细胞粘附和细胞增殖长达21天。我们的结果强调了PCL / fND纳米复合材料作为智能生物支架的巨大潜力，可用于先进的生物医学应用，例如组织工程，生物传感和治疗学。