ABSTRACT

This study evaluated the possibility of using Oocystis borgei to prevent and control harmful algae blooms. Firstly, Microcystis aeruginosa and O. borgei were co-cultured to assess the competition for nutrients between them. Different physiological and biochemical parameters, such as growth, cell membrane permeability and esterase activities were determined in exudate culture experiment to investigate allelopathic effects of O. borgei culture and mixed cultures (O. borgei and M. aeruginosa) at different growth phase on harmful microalgae (M. aeruginosa). Results showed that O. borgei could significantly inhibited M. aeruginosa when volume ratios were 4:1 and 1:1 (M. aeruginosa: O. borgei) in co-culture experiment. Further, it was found that the membrane system of M. aeruginosa was disintegrated by the culture filtrate of O. borgei at exponential phase. In addition, esterase activities and photorespiration were significantly inhibited. In conclusion, O. borgei exhibited different allelopathic effects at different growth phase. Its exponential phase showed a significant inhibitory effect, while no inhibitory effect was observed at the decline phase.

摘要

本研究评估了使用博氏卵囊来预防和控制有害藻类大量繁殖的可能性。首先，共培养铜绿微囊藻和博尔氏菌，以评估它们之间的营养竞争。在渗出液培养实验中测定了不同的生理生化参数，如生长、细胞膜通透性和酯酶活性，以研究不同生长阶段的博尔盖菌培养物和混合培养物（博尔盖菌和铜绿假单胞菌）对有害微藻的化感作用。（绿脓杆菌）。结果表明，O. borgei能显着抑制M. aeruginosa当体积比为 4:1 和 1:1 ( M. aeruginosa: O. borgei ) 时在共培养实验中。此外，还发现绿脓杆菌的膜系统在指数期被博氏杆菌的培养滤液分解。此外，酯酶活性和光呼吸作用受到显着抑制。总之，O. borgei在不同的生长阶段表现出不同的化感作用。其指数期表现出显着的抑制作用，而下降期未观察到抑制作用。