Assessing mitochondrial function in cell-based systems is a central component of metabolism research. However, the selection of an initial measurement technique may be complicated given the range of parameters that can be studied as well as the need to define the mitochondrial (dys)function of interest. This methods-focused review compares and contrasts the use of mitochondrial membrane potential measurements, plate-based respirometry, and metabolomics and stable isotope tracing. We demonstrate how measurements of (i) cellular substrate preference, (ii) respiratory chain activity, (iii) cell activation, and (iv) mitochondrial biogenesis are enriched by integrating information from multiple methods. This manuscript is meant to serve as a perspective to help choose which technique might be an appropriate initial method to answer a given question, as well as provide a broad 'roadmap' for designing follow-up assays to enrich datasets or resolve ambiguous results.

中文翻译：

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力、通量和燃料：通过整合膜电位、呼吸和代谢物的测量来跟踪线粒体代谢

评估基于细胞的系统中的线粒体功能是代谢研究的核心组成部分。然而，考虑到可以研究的参数范围以及定义感兴趣的线粒体 (dys) 功能的需要，初始测量技术的选择可能会很复杂。这篇以方法为重点的评论比较和对比了线粒体膜电位测量、基于平板的呼吸测定法、代谢组学和稳定同位素追踪的使用。我们展示了如何通过整合来自多种方法的信息来丰富 (i) 细胞底物偏好、(ii) 呼吸链活性、(iii) 细胞活化和 (iv) 线粒体生物发生的测量。这份手稿旨在作为一个视角，帮助选择哪种技术可能是回答给定问题的合适初始方法，