The aim of this work was to assess the antimicrobial efficacy on Pseudomonas aeruginosa of nanomicelles loaded with curcumin (CUR) alone and activated by blue laser light in an antimicrobial photodynamic therapy (APDT) approach. First, free CUR in liquid suspension and loaded in three amphiphilic nanomicelles (CUR‐DAPMA, CUR‐SPD and CUR‐SPM) were tested both on bacteria and keratinocytes. While free CUR exerted limited efficacy showing moderate cytotoxicity, a strong inhibition of bacterial growth was obtained using all three nanosystems without toxicity on eukaryotic cells. CUR‐SPM emerged as the most effective, and was therefore employed in APDT experiments. Among the three sublethal blue laser (λ 445 nm) protocols tested, the ones characterized by a fluence of 18 and 30 J/cm² further decreased the antimicrobial concentration to 50 nM. The combination of blue laser APDT with CUR‐SPM nanomicelles results in an effective synergistic activity that represents a promising novel therapeutic approach on resistant species.

中文翻译：

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载有姜黄素的两亲纳米胶束单独对铜绿假单胞菌具有抗菌活性，并被蓝色激光激活

这项工作的目的是评估抗菌素光动力学疗法（APDT）方法中单独加载姜黄素（CUR）并被蓝色激光激活的纳米胶束对铜绿假单胞菌的抗菌功效。首先，对细菌和角质形成细胞均测试了液体悬浮液中的游离CUR，并加载了三个两亲纳米胶束（CUR-DAPMA，CUR-SPD和CUR-SPM）。尽管游离的CUR发挥有限的功效，显示出中等的细胞毒性，但是使用这三种纳米系统均对细菌的生长产生了强烈的抑制作用，而对真核细胞没有毒性。CUR-SPM成为最有效的方法，因此被用于APDT实验中。在测试的三种亚致死蓝色激光（λ445 nm）方案中，特征在于注量分别为18 J / cm ²和30 J / cm ²进一步将抗菌素浓度降低至50 nM。蓝色激光APDT与CUR-SPM纳米胶束的结合产生了有效的协同活性，代表了一种有前途的新型抗药性治疗方法。