In this study, four genes encoding secondary acyltransferases of lipid A in Vibrio parahaemolyticus ATCC33846 were identified. When the four genes were overexpressed in Escherichia coli MLK1067 that which produces the penta-acylated lipid A lacking the secondary acylation at the C3′ position, a C_12:0 secondary acyl chain was added at the C3′ position of lipid A only in E. coli overexpressing VP_RS01045, but not VP_RS00880, VP_RS08405, or VP_RS12170. When the four genes were overexpressed in E. coli MKV15b that produces lipid IV_A, a C_12:0 secondary acyl chain was again added at the C3′ position in E. coli overexpressing VP_RS01045, but a C_14:0 secondary acyl chain was added at the C2′ position of lipid A in E. coli overexpressing VP_RS00880, VP_RS08405, or VP_RS12170. The results indicate that four acyltransferases of lipid A are encoded by VP_RS01045, VP_RS00880, VP_RS08405, or VP_RS12170 in V. parahaemolyticus. The acyltransferase encoded by VP_RS01045 adds a C_12:0 secondary acyl chain at the C3′ position of lipid A, whereas the acyltransferase encoded by VP_RS00880, VP_RS08405, or VP_RS12170 adds a C_14:0 secondary acyl chain at the C2′ position of lipid A. This work contributes to understanding the biosynthetic pathway of lipid A in V. parahaemolyticus.

中文翻译：

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副溶血性弧菌脂质 A 生物合成的四种二级酰基转移酶的鉴定

在这项研究中，鉴定了在副溶血性弧菌ATCC33846 中编码脂质 A 二级酰基转移酶的四个基因。当这四个基因在大肠杆菌MLK1067 中过表达时，产生在 C3' 位置缺乏二级酰化的五酰化脂质 A，仅在E中脂质 A 的 C3' 位置添加 C _{12:0二级酰基链}. 大肠杆菌过度表达VP_RS01045，但不表达 VP_RS00880 、VP_RS08405或VP_RS12170。当这四个基因在产生脂质 IV _A的大肠杆菌MKV15b 中过表达时，a C _12:0在过表达VP_RS01045的大肠杆菌的 C3' 位置再次添加了二级酰基链，但在过表达VP_RS00880、VP_RS08405或VP_RS12170的大肠杆菌的脂质 A 的 C2' 位置添加了 C _14:0二级酰基链。结果表明，副溶血性弧菌中脂质A的四种酰基转移酶分别由VP_RS01045 、 VP_RS00880 、 VP_RS08405或VP_RS12170编码。VP_RS01045编码的酰基转移酶加了一个C _12:0二级酰基链位于脂质 A 的 C3' 位置，而由VP_RS00880、VP_RS08405或VP_RS12170编码的酰基转移酶在脂质 A 的 C2' 位置添加了一条 C _14:0二级酰基链。这项工作有助于了解脂质 A 的生物合成途径副溶血性弧菌中的脂质A。