ABSTRACT This study aimed to verify the effects of short-term supplementation of diets rich in lipids or glycogen precursors on the follicular environment, oocyte mitochondrial gene expression, and in vitro embryo development. Thirty adult Anglo-Nubian multiparous crossbred goats were fed for 28 days with a total mixed ration (TMR) to satisfy the requirements of breeding for adult non-dairy goats. Seven days prior to the end of the feeding period, either ground flaxseed on a 10.5% DM basis (Flaxseed Group - FG, n = 10), or a 200-mL/goat/day solution of crude glycerin in water at a 9:1 ratio (80% Glycerin Group - GG, n = 10) were added to the TMR diet. The Control Group (CG) was maintained at the TMR diet (n = 10). Ovaries were collected upon slaughter for collection of follicular contents. Glucose, cholesterol and triglyceride concentrations and the glutathione peroxidase activity were determined in the follicular fluid, and the expression of Cox-1, NRF-1, PPARGC1-A and GDF-9 genes performed in granulosa cells and in oocytes by qPCR. Viable COCs were in vitro-matured for 24 h, to be parthenogenetically activated and in vitro-cultured for seven days up to the blastocyst stage. Intra-follicular glucose concentration was higher in the Glycerin (20.2 ± 2.5 mg\dL GG vs. 5.6 ± 1.7 mg\dL FG vs. 14.2 ± 3.5 mg\dL CG; P

中文翻译：

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短期补充富含脂质或糖原前体的饮食会影响山羊孤雌生殖后的卵泡内环境、卵母细胞线粒体基因表达和胚胎发育

摘要 本研究旨在验证短期补充富含脂质或糖原前体的饮食对卵泡环境、卵母细胞线粒体基因表达和体外胚胎发育的影响。30只成年盎格鲁-努比亚经产杂交山羊用全混合日粮（TMR）喂养28天，以满足成年非奶山羊的繁殖要求。饲喂期结束前 7 天，以 10.5% DM 为基础（亚麻籽组 - FG，n = 10）研磨亚麻籽，或 200 毫升/山羊/天的粗甘油水溶液，浓度为 9：将 1 个比例（80% 甘油组 - GG，n = 10）添加到 TMR 饮食中。对照组 (CG) 维持 TMR 饮食 (n = 10)。在屠宰时收集卵巢以收集卵泡内容物。葡萄糖，在卵泡液中测定胆固醇和甘油三酯浓度以及谷胱甘肽过氧化物酶活性，并通过 qPCR 在颗粒细胞和卵母细胞中进行 Cox-1、NRF-1、PPARGC1-A 和 GDF-9 基因的表达。活的 COC 在体外成熟 24 小时，孤雌生殖激活并在体外培养 7 天直至囊胚阶段。甘油中的卵泡内葡萄糖浓度较高（20.2 ± 2.5 mg\dL GG vs. 5.6 ± 1.7 mg\dL FG vs. 14.2 ± 3.5 mg\dL CG；P