Glyphosate (Gly) is the herbicide widely used in agricultural fields and landscaping. Mammalian exposure to glyphosate could cause neurotoxicity, blood, liver, kidney, endocrine, reproductive, genetic and other toxic effects. Melatonin (MT) is a neuroendocrine hormone secreted mainly by the pineal gland. It is unknown whether MT can improve reproductive defects and hormonal disorders in mice exposed to Gly. In this study, mice were exposed to 250 and 500 mg/kg Gly by intragastric administered and 15 mg/kg MT was treated via intraperitoneal injection for 7 days. Our results shown that the weight gain of mice, the ovarian coefficient and the ATP content in the ovaries were decreased significantly. Gly-treated oocytes showed that the first polar body extrusion failed, the level of oxidative stress increased, and the mitochondrial membrane potential (MMP) decreased. Subsequently, our results showed that increased expression level of BAX protein, reduced expression of BCL-2 protein, ATG12 and LC3 protein expression increased in ovaries after Gly treatment. At the same time, Gly exposure led to abnormal expression of Hypothalamic-Pituitary-Thyroid (HPT) axis-related genes and disrupted hormone homeostasis. After the injection of 15 mg/kg MT, the oocytes showed decreased oxidative stress level, increased mitochondrial membrane potential, incremental ATP content in the ovaries and the hormone levels were approached to the control group. Thus, our results demonstrated that melatonin can improve oocyte quality and maintain hormone homeostasis in mice exposed to glyphosate.

草甘膦（Gly）是一种广泛用于农田和园林绿化的除草剂。哺乳动物接触草甘膦可能导致神经毒性、血液、肝脏、肾脏、内分泌、生殖、遗传和其他毒性影响。褪黑激素 (MT) 是一种主要由松果体分泌的神经内分泌激素。尚不清楚 MT 是否可以改善暴露于 Gly 的小鼠的生殖缺陷和激素紊乱。在这项研究中，小鼠通过胃内给药暴露于 250 和 500 mg/kg Gly，并通过腹腔注射治疗 15 mg/kg MT 7 天。我们的结果表明，小鼠体重增加、卵巢系数和卵巢中的ATP含量显着降低。甘氨酸处理的卵母细胞显示第一次极体挤出失败，氧化应激水平升高，线粒体膜电位（MMP）降低。随后，我们的结果表明，Gly 处理后卵巢中 BAX 蛋白表达水平增加，BCL-2 蛋白表达减少，ATG12 和 LC3 蛋白表达增加。同时，Gly 暴露导致下丘脑-垂体-甲状腺 (HPT) 轴相关基因的异常表达和激素稳态的破坏。注射15mg/kg MT后，卵母细胞氧化应激水平降低，线粒体膜电位增加，卵巢中ATP含量增加，激素水平接近对照组。因此，我们的结果表明，褪黑激素可以改善暴露于草甘膦的小鼠的卵母细胞质量并维持激素稳态。我们的结果表明，Gly处理后卵巢中BAX蛋白表达水平增加，BCL-2蛋白表达减少，ATG12和LC3蛋白表达增加。同时，Gly 暴露导致下丘脑-垂体-甲状腺 (HPT) 轴相关基因的异常表达和激素稳态的破坏。注射15mg/kg MT后，卵母细胞氧化应激水平降低，线粒体膜电位增加，卵巢中ATP含量增加，激素水平接近对照组。因此，我们的结果表明，褪黑激素可以改善暴露于草甘膦的小鼠的卵母细胞质量并维持激素稳态。我们的结果表明，Gly处理后卵巢中BAX蛋白表达水平增加，BCL-2蛋白表达减少，ATG12和LC3蛋白表达增加。同时，Gly 暴露导致下丘脑-垂体-甲状腺 (HPT) 轴相关基因的异常表达和激素稳态的破坏。注射15mg/kg MT后，卵母细胞氧化应激水平降低，线粒体膜电位增加，卵巢中ATP含量增加，激素水平接近对照组。因此，我们的结果表明，褪黑激素可以改善暴露于草甘膦的小鼠的卵母细胞质量并维持激素稳态。Gly 暴露导致下丘脑-垂体-甲状腺 (HPT) 轴相关基因的异常表达和激素稳态的破坏。注射15mg/kg MT后，卵母细胞氧化应激水平降低，线粒体膜电位增加，卵巢中ATP含量增加，激素水平接近对照组。因此，我们的结果表明，褪黑激素可以改善暴露于草甘膦的小鼠的卵母细胞质量并维持激素稳态。Gly 暴露导致下丘脑-垂体-甲状腺 (HPT) 轴相关基因的异常表达和激素稳态的破坏。注射15mg/kg MT后，卵母细胞氧化应激水平降低，线粒体膜电位增加，卵巢中ATP含量增加，激素水平接近对照组。因此，我们的结果表明，褪黑激素可以改善暴露于草甘膦的小鼠的卵母细胞质量并维持激素稳态。