Engineered stimuli-responsive drug delivery strategies grasp enormous potential in biomedical applications for disease treatment due to their exploited therapeutic efficiency. In the current study, we developed poly 4-hydroxyphenyl methacrylate-carbon nano-onions (PHPMA-CNOs = f-CNOs) embedded bovine serum albumin (BSA) nanocomposite fibers by Forcespinning® (FS) technology for stimuli-responsive release of cargo, using doxorubicin (DOX) as a model drug. Nanocomposite fiber system showed thermosensitive drug release and exhibited around 72 and 95% of drug release at 37 and 43 °C, respectively. A slow and prolonged DOX release was observed over a 15-day study. The amount of drug released was determined by the concentration of the DOX payload, incubation temperature, and pH of the released medium. Owing to the f-CNOs incorporation, the mechanical strength (18.23 MPa) of hybrid BSA nanocomposite fibers was enhanced significantly. Besides, in vitro degradation, water contact angles, and thermal properties of nanocomposite fibers have augmented. During the in vitro cytotoxicity assessment, nanocomposite fibers exhibited improved cell viability against human fibroblast cells. Nonetheless, the external-stimuli-dependent and sustained DOX release perhaps reduces its circumventing side effects and show potential applications in biomedical research.

工程化的刺激响应性药物递送策略因其开发的治疗效率而在生物医学应用中具有巨大的潜力，可用于疾病治疗。在当前的研究中，我们通过Forcespinning®（FS）技术开发了聚甲基丙烯酸4-羟基苯酯-碳纳米洋葱（PHPMA-CNOs = f-CNOs）嵌入的牛血清白蛋白（BSA）纳米复合纤维，用于刺激性释放货物，使用阿霉素（DOX）作为模型药物。纳米复合纤维系统显示出热敏性药物释放，并分别在37和43°C下显示出约72％和95％的药物释放。在为期15天的研究中，观察到DOX释放缓慢而延长。释放的药物量取决于DOX有效负载的浓度，孵育温度和释放介质的pH。由于加入了f-CNO，杂化BSA纳米复合纤维的机械强度（18.23 MPa）明显提高。此外，纳米复合纤维的体外降解，水接触角和热性能得到增强。在体外细胞毒性评估中，纳米复合纤维对人成纤维细胞表现出改善的细胞活力。尽管如此，依赖外部刺激并持续释放DOX可能会降低其规避的副作用，并显示出在生物医学研究中的潜在应用。