Micromonospora craniellae LHW63014^T is a novel marine Micromonospora, isolated from a Craniella species sponge collected in the South China Sea. In this study, we report the complete genome sequence of M. craniellae LHW63014^T, which is comprised of a circular chromosome of 6,839,926 bp with the G + C content of 70.9 mol%. The complete genome contained 6572 protein-coding genes, 48 tRNA genes, and 9 rRNA genes. Genomic annotations revealed that 79.09% of the protein-coding genes were assigned to the COG database, among which, the abundant genes were predicted to be involved in transcription, replication, recombination and repair, and amino acid transport and metabolism. Secondary metabolites prediction using antiSMASH revealed that 22 biosynthetic gene clusters (BGC) of secondary metabolites were located in the genome of M. craniellae LHW63014^T, 19 of which showed low similarity (<50%) to known BGCs and 5 of which showed the closest homology with BGCs encoding metal ion-chelating agents, indicating the immense potential of M. craniellae LHW63014^T to produce a wide variety of novel antibiotics, especially for metal ion-chelating agents.

Micromonospora craniellae LHW63014 ^T是一种新型海洋小单孢菌，分离自在南海收集的Craniella种海绵。在这项研究中，我们报告了M. craniellae LHW63014 ^T的完整基因组序列，它由6,839,926 bp的环状染色体组成，G + C含量为70.9 mol％。完整的基因组包含6572个蛋白质编码基因、48个tRNA基因和9个rRNA基因。基因组注释显示，79.09%的蛋白质编码基因被归入COG数据库，其中预测丰富的基因参与转录、复制、重组和修复以及氨基酸转运和代谢。使用 antiSMASH 的次级代谢产物预测显示，22 个次级代谢产物的生物合成基因簇 (BGC) 位于M. craniellae LHW63014 ^T的基因组中，其中 19 个与已知 BGC 显示出低相似性（<50%），其中 5 个与编码金属离子螯合剂的BGC显示出最接近的同源性，表明M. craniellae LHW63014 ^T具有生产多种新型抗生素的巨大潜力，尤其适用于金属离子螯合剂。